Sheep Thrombin Polyclonal Antibody

Sheep anti-rabbit Thrombin, Affinity-Purified IgG

Cat. Num. AAA512158

• Purity: Affinity purified IgG.; Vial containing ml of IgG purified by chromatography on immobilized PPACK-thrombin. Total protein is 0.5 mg.
• Synonyms: Thrombin; Polyclonal Antibody; Sheep anti-rabbit Thrombin; Affinity-Purified IgG; rabbit; anti-Thrombin antibody

• Host: Sheep
• Clonality: Polyclonal
• Specificity: This antibody is specific for rabbit thrombin (or prothrombin) as demonstrated by immunoelectrophoresis and ELISA.
• Purity/Purification: Affinity purified IgG.; Vial containing ml of IgG purified by chromatography on immobilized PPACK-thrombin. Total protein is 0.5 mg.
• Form/Format: Affinity-purified IgG (APIgG), clear liquid.
• Concentration: APIgG concentration is mg/ml, determined by absorbance using an extinction coefficient (E1%280) of 13.4. (varies by lot)

• Application Notes: Suitable as a source of antibodies to rabbit thrombin (and prothrombin).
• Immunogen: Thrombin prepared from purified rabbit prothrombin, active site blocked with PPACK.
• Buffer: 10 mM HEPES, pH 7.4, 150 mM NaCl, 50% (v/v) glycerol.
• Neutralizing Activity: Not determined
• Preparation and Storage: Store between -10 and -20 degree C. Product will become viscous but will not freeze. Avoid storage in frost-free freezers. Keep vial tightly capped. Allow product to warm to room temperature and gently mix before use.

Related Product Information for anti-Thrombin antibody

Thrombin (EC3.4.21.5, alpha-thrombin) is the product of proteolytic activation of the zymogen prothrombin. Human thrombin is a two-chain serine protease with a mass of 37 kDa. The active site is located within the heavy chain. Thrombin has a high specificity for certain arginine bonds in protein substrates. The primary substrate is fibrinogen which thrombin converts to fibrin through the cleavage of four arginyl-glycyl peptide bonds. Thrombin is also an important activator of platelets, factor XIII, Protein C and TAFI (Plasma procarboxypeptidase B). In a positive feedback mechanism, thrombin increases the rate of its own production by activation of factors VIII and V. The rate of thrombin production is subsequently limited indirectly through the activation of Protein C by thrombin, which then inactivates the activated cofactors VIII and V. The binding of thrombin to thrombomodulin on the cell surface dramatically alters thrombin's specificity, increasing it's activity toward Protein C and TAFI, and decreasing it's activity toward fibrinogen and activating cofactors VIII and V. In plasma, thrombin activity is inhibited primarily by antithrombin and to a lesser extent heparin cofactor II. The rate of inhibition by both of these inhibitors is profoundly increased in the presence of optimal concentrations of heparin. Other physiological inhibitors of thrombin in the absence of heparin include alpha2-macroglobulin and alpha1-antitrypsin1-4.

References

1. Mann KG; Prothrombin and Thrombin; in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 184-199, J.B. Lippincott Co., Philadelphia PA, USA, 1994.
2. Stubbs MT, Bode W; A Player of Many Parts: The Spotlight Falls on Thrombin's Structure; Thrombosis Research 69, pp 1-58, 1993.
3. Downing MW, Bloom JW, Mann KG; Comparison of the Inhibition of Thrombin by Three Plasma Protease Inhibitors; Biochemistry 17, pp 2649- 2653, 1978.
4. Weitz JI, Hudoba M, Massel D, Maranganore J, Hirsh J; Clot-bound Thrombin is Protected from Inhibition by Heparin-ATIII but is Susceptible to - ATIII independent Inhibitors; J. Clin. Invest 86, pp 385-391, 1990.

NCBI and Uniprot Product Information

• NCBI GI #: 213487
• NCBI Official Full Name: thrombin
• Protein Family: Thrombin