Goat alpha2-Antiplasmin Polyclonal Antibody | anti-Serpinf2 antibody

Goat anti-human alpha2Antiplasmin (alpha2AP), Affinity-Purified IgG

Cat. Num. AAA512007

• Gene Names: Serpinf2; Pli; AI747498; Serpimf2
• Purity: Affinity purified IgG.; Vial containing ml of IgG purified by affinitychromatography on immobilized alpha2AP. Total protein is 0.5 mg.
• Synonyms: alpha2-Antiplasmin; Polyclonal Antibody; Goat anti-human alpha2Antiplasmin (alpha2AP); Affinity-Purified IgG; human; anti-Serpinf2 antibody

• Host: Goat
• Clonality: Polyclonal
• Specificity: This antibody is specific for alpha2AP as demonstrated by immunoelectrophoresis and ELISA.
• Purity/Purification: Affinity purified IgG.; Vial containing ml of IgG purified by affinitychromatography on immobilized alpha2AP. Total protein is 0.5 mg.
• Form/Format: Affinity-purified IgG (APIgG), clear liquid.
• Concentration: APIgG concentration is mg/ml, determined by absorbance using an extinction coefficient (E1%280) of 13.4. (varies by lot)

• Application Notes: Suitable as a source of enriched antibodies to human alpha2AP.
• Immunogen: Human alpha2antiplasmin purified from plasma.
• Buffer: 10 mM HEPES, pH 7.4, 150 mM NaCl, 50% (v/v) glycerol.
• Neutralizing Activity: Not determined
• Preparation and Storage: Store between -10 and -20 degree C. Product will become viscous but will not freeze. Avoid storage in frost-free freezers. Keep vial tightly capped. Allow product to warm to room temperature and gently mix before use.

Related Product Information for anti-Serpinf2 antibody

alpha2Antiplasmin (alpha2AP), also known as alpha2Plasmin Inhibitor (alpha2PI), is a member of the SERPIN family of proteinase inhibitors and the primary inhibitor of the enzyme plasmin in blood. It is produced in the liver and circulates in plasma at ~70 ug/ml (~1 uM). alpha2AP is a single chain molecule with a mass of 67 kDa as determined by SDS-PAGE. The primary target enzyme for alpha2AP is plasmin, but alpha2AP also acts as secondary or "backup" inhibitor of activated F.XI, activated Protein C and trypsin. Inhibition of these enzymes by alpha2AP occurs by proteolytic cleavage after Arg364 with subsequent rapid formation of a stable, inactive 1:1 enzyme-alpha2AP complex. alpha2AP also acts to regulate fibrinolysis by binding to the lysine binding sites on plasminogen thus competitively inhibiting plasminogen binding to fibrin. About 30% of alpha2AP present in plasma is partially degraded and lacks a peptide in the carboxyl region that contains the plasminogen-binding site. This form of alpha2AP (~65 kDa) has a reduced rate of plasmin inhibition and has been referred to as the "slow form" of alpha2AP. During fibrin formation, a portion of circulating alpha2AP is cross-linked to the alpha-chain of fibrin by activated factor XIII, and this linking of plasmin inhibitor to the plasmin substrate provides an additional measure of protection to the fibrin clot from proteolysis by plasmin1-4.

References

1. Aoki N, Suni Y, Miura O, Hirosawa S; Human alpha2Plasmin Inhibitor; Methods in Enzymology, 223, pp 185-197, 1993.
2. Shieh BH, Travis J; The Reactive Site of Human alpha2-Plasmin Inhibitor; JBC 262, pp 6055-6059, 1987.
3. Moroi M, Aoki N; Isolation and Characterization of alpha2-Plasmin Inhibitor from Human Plasma; JBC 251, pp 5956-5965, 1976.
4. Harpel PC; Blood Proteolytic Enzyme Inhibitors: Their Role in Modulating Blood Coagulation and Fibrinolytic Enzyme Pathways; in Hemostasis and Thrombosis, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 738-747, J.B. Lippincott Co., Philadelphia PA, USA, 1982.

NCBI and Uniprot Product Information

• NCBI GI #: 6679383
• NCBI GeneID: 18816
• NCBI Accession #: NP_032904.1
• NCBI GenBank Nucleotide #: NM_008878.2
• UniProt Accession #: Q5ND36; Q5ND35; Q61247; Q6LAK8
• Molecular Weight: 54,972 Da
• NCBI Official Full Name: alpha-2-antiplasmin
• NCBI Official Synonym Full Names: serine (or cysteine) peptidase inhibitor, clade F, member 2
• NCBI Official Symbol: Serpinf2
• NCBI Official Synonym Symbols: Pli; AI747498; Serpimf2
• NCBI Protein Information: alpha-2-antiplasmin; serpin F2; alpha-2-AP; alpha-2-PI; OTTMUSP00000006499; OTTMUSP00000006500; alpha 2 antiplasmin; alpha-2-plasmin inhibitor; plasmin inhibitor alpha 2; pigment epithelium derived factor; serine (or cysteine) proteinase inhibitor, clade F, member 2
• UniProt Protein Name: Serine (Or cysteine) peptidase inhibitor, clade F, member 2, isoform CRA_c
• Protein Family: Alpha-2-antiplasmin
• UniProt Gene Name: Serpinf2
• UniProt Entry Name: Q5ND36_MOUSE

Uniprot Description

Sequence similarities: Belongs to the serpin family. RuleBase RU000411