Western Blot (WB)
(Western blot of NIH 3T3 cell lysate showing specific immunolabeling of the ~35 kDa SOX2 protein.)
Rabbit SOX2 Polyclonal Antibody | anti-SOX2 antibody
SOX2
Gene Names
SOX2; ANOP3; MCOPS3
Reactivity
Reactivity assumed based on 100% sequence homology: Human
Applications
Western Blot
Purity
Antigen Affinity Purified from Pooled Serum
Synonyms
SOX2; Polyclonal Antibody; anti-SOX2 antibody
Host
Rabbit
Reactivity
Reactivity assumed based on 100% sequence homology: Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified from Pooled Serum
Form/Format
100ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100ug BSA per ml and 50% glycerol.
Applicable Applications for anti-SOX2 antibody
Western Blot (WB)
Application Notes
WB: 1:1000
ICC: 1:20
ICC: 1:20
Species Positively Tested
Mouse
Immunogen Species
Human
Immunogen Information
Fusion protein from the C-terminal region of human SOX2 protein
Antigen
Fusion protein from the C-terminal region of human SOX2 protein.
Preparation and Storage
Storage at -20 degree C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20 degree C.
Shipping: Blue Ice
Country of Origin: USA
Shipping: Blue Ice
Country of Origin: USA
Western Blot (WB)
(Western blot of NIH 3T3 cell lysate showing specific immunolabeling of the ~35 kDa SOX2 protein.)
Western Blot (WB)
(Western blot of NIH 3T3 cell lysate showing specific immunolabeling of the ~35 kDa SOX2 protein.)
Testing Data
(DYKDDDDK-tagged SOX2 transfected COS cells showing SOX2 staining in red (Cat.# MBS502239, 1:20) and DYKDDDDK staining in green, causing positive transfected cells to appear yellow. DNA is stained blue with DAPI.)
Testing Data
(DYKDDDDK-tagged SOX2 transfected COS cells showing SOX2 staining in red (Cat.# MBS502239, 1:20) and DYKDDDDK staining in green, causing positive transfected cells to appear yellow. DNA is stained blue with DAPI.)
Related Product Information for anti-SOX2 antibody
SOX2 (SRY (sex-determining region Y)-box 2) is one of 20 members of the Sox family proteins that play essential roles in cell differentiation, development, and organogenesis. SOX2, a group B Sox family protein, is one of the master transcriptional factors, along with Oct3/4 (Nichols, J., et al, 1998) and PAK6 (Kamachi, Y. et al, 2001) that are important in maintaining the pluripotency of embryonic stem cells, thus making SOX2 an excellent marker of neural stem cells. SOX2 has also been identified as a lineage-survival oncogene in lung and esophageal squamous cell carcinomas due to peak amplification on chromosome 3q26.33 which contains the SOX2 gene transcription factor (Bass, A.J., et al, 2009). Additionally, Sox2 has been reported to be highly deregulated in glioblastomas (Gangemi, R. M. R, et al, 2009) and breast cancer (Rodriguez-Pinilla, S.M., et al, 2007).
Product Categories/Family for anti-SOX2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34kDa
NCBI Official Full Name
transcription factor SOX-2
NCBI Official Synonym Full Names
SRY (sex determining region Y)-box 2
NCBI Official Symbol
SOX2
NCBI Official Synonym Symbols
ANOP3; MCOPS3
NCBI Protein Information
transcription factor SOX-2; transcription factor SOX2; SRY-related HMG-box gene 2
UniProt Protein Name
Transcription factor SOX-2
UniProt Gene Name
SOX2
UniProt Entry Name
SOX2_HUMAN
Similar Products
Product Notes
The SOX2 sox2 (Catalog #AAA502239) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SOX2 reacts with Reactivity assumed based on 100% sequence homology: Human and may cross-react with other species as described in the data sheet. AAA Biotech's SOX2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000 ICC: 1:20. Researchers should empirically determine the suitability of the SOX2 sox2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SOX2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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