Mouse Visinin-Like Protein 1 (VSNL1) Monoclonal Antibody | anti-VSNL1 antibody

Anti-Visinin-Like Protein 1 (VSNL1)

Cat. Num. AAA500046

• Gene Names: VSNL1; HLP3; VILIP; HPCAL3; HUVISL1; VILIP-1
• Reactivity: Bovine, human, mouse, rat
• Applications: Western Blot, Immunofluorescence
• Purity: Affinity Purified (Affinity purified from tissue culture supernatant.)
• Synonyms: Visinin-Like Protein 1 (VSNL1); Monoclonal Antibody; Anti-Visinin-Like Protein 1 (VSNL1); anti-VSNL1 antibody

• Host: Mouse
• Reactivity: Bovine, human, mouse, rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 2D11
• Specificity: Specific for the ~22k protein
• Purity/Purification: Affinity Purified (Affinity purified from tissue culture supernatant.)
• Form/Format: 100 ul liquid containing 10 mM sodium azide.
• Sequence Length: 191

• Applicable Applications for anti-VSNL1 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:2,000; IF: 1: 500
• Antigen: Recombinant full length human VSNL1 expressed in and purified from E. Coli.
• Immunogen Information: Recombinant human VSNL1 purified from E. coli
• Immunogen Species: Human
• Species Reactivity Note: The antibody has been directly tested for reactivity in bovine, human, mouse and rat.
• Biological Significance: Visinin-like protein 1 (VSNL1), also known as VILIP1, is a calcium sensor protein expressed exclusively in neurons. Highest levels of VSNL1 expression are found in cerebellar Purkinje cells. VSNL1 has been implicated in the modulation of cell signaling cascades via regulation of adenyl cyclase activity (Braunewell et al., 1997). Additionally, VSNL1 has been associated with amyloid plaques and neurofibrillar tangles in Alzheimer's disease (Schnurra et al., 2001).
• Preparation and Storage: Store at -20 degree C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.

Testing Data

Related Product Information for anti-VSNL1 antibody

Mouse monoclonal antibody

References

• Braunewell KH, Spilker C, Behnisch T, Gundelfinger ED (1997) The neuronal calcium-sensor protein VILIP modulates cyclic AMP accumulation in stably transfected C6 glioma cells: amino-terminal myristoylation determines functional activity. J. Neurochem. 68 (5): 2129-39.
• Schnurra I, Bernstein HG, Riederer P, Braunewell KH (2002) The neuronal calcium sensor protein VILIP-1 is associated with amyloid plaques and extracellular tangles in Alzheimer's disease and promotes cell death and tau phosphorylation in vitro: a link between calcium sensors and Alzheimer's disease. Neurobiol. Dis. 8 (5): 900-9.

NCBI and Uniprot Product Information

• NCBI GI #: 21361559
• NCBI GeneID: 7447
• NCBI Accession #: NP_003376.2
• NCBI GenBank Nucleotide #: NM_003385.4
• UniProt Accession #: P62760; P28677; P29103; P42323; Q9UM20; D6W515
• Molecular Weight: 22
• NCBI Official Full Name: visinin-like protein 1
• NCBI Official Synonym Full Names: visinin-like 1
• NCBI Official Symbol: VSNL1
• NCBI Official Synonym Symbols: HLP3; VILIP; HPCAL3; HUVISL1; VILIP-1
• NCBI Protein Information: visinin-like protein 1; VLP-1; hippocalcin-like protein 3
• UniProt Protein Name: Visinin-like protein 1
• Protein Family: Visinin-like protein
• UniProt Gene Name: VSNL1
• UniProt Synonym Gene Names: VISL1; VILIP; VLP-1; HLP3
• UniProt Entry Name: VISL1_HUMAN

NCBI Description

This gene is a member of the visinin/recoverin subfamily of neuronal calcium sensor proteins. The encoded protein is strongly expressed in granule cells of the cerebellum where it associates with membranes in a calcium-dependent manner and modulates intracellular signaling pathways of the central nervous system by directly or indirectly regulating the activity of adenylyl cyclase. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Regulates (in vitro) the inhibition of rhodopsin phosphorylation in a calcium-dependent manner

By similarity.

Tissue specificity: Brain and retina. Neuron-specific in the central and peripheral nervous system. Increased in the cerebrospinal fluid of Alzheimer disease patients (at protein level). Ref.6

Miscellaneous: Probably binds three calcium ions.

Sequence similarities: Belongs to the recoverin family.Contains 4 EF-hand domains.