Rat Excitatory Amino Acid Transporter 1 ELISA Kit | EAAT1 elisa kit

Rat Excitatory Amino Acid Transporter 1 ELISA Kit

Cat. Num. AAA50004

• Gene Names: SLC1A3; EA6; EAAT1; GLAST; GLAST1
• Reactivity: Rat
• Synonyms: Excitatory Amino Acid Transporter 1; Rat Excitatory Amino Acid Transporter 1 ELISA Kit; EAAT1 elisa kit

• Reactivity: Rat
• Specificity: No significant cross-reactivity or interference between Rat EAAT1 and analogues was observed.
• Sequence Length: 496

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 0.25 ng/ml - 8 ng/ml.
• Sensitivity: 0.1 ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated EAAT1 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat EAAT1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add EAAT1 and EAAT1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of EAAT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for EAAT1 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated EAAT1 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat EAAT1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add EAAT1 and EAAT1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of EAAT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 584277111
• NCBI GeneID: 6507
• NCBI Accession #: NP_001276868.1
• NCBI GenBank Nucleotide #: NM_001289939.1
• UniProt Accession #: P43003; Q4JCQ8; B2R5T3
• Molecular Weight: 59,572 Da
• NCBI Official Full Name: excitatory amino acid transporter 1 isoform 4
• NCBI Official Synonym Full Names: solute carrier family 1 (glial high affinity glutamate transporter), member 3
• NCBI Official Symbol: SLC1A3
• NCBI Official Synonym Symbols: EA6; EAAT1; GLAST; GLAST1
• NCBI Protein Information: excitatory amino acid transporter 1; GLAST-1; solute carrier family 1 member 3; sodium-dependent glutamate/aspartate transporter 1
• UniProt Protein Name: Excitatory amino acid transporter 1
• Protein Family: Excitatory amino acid transporter
• UniProt Gene Name: SLC1A3
• UniProt Synonym Gene Names: EAAT1; GLAST; GLAST1; GLAST-1
• UniProt Entry Name: EAA1_HUMAN

NCBI Description

This gene encodes a member of a member of a high affinity glutamate transporter family. This gene functions in the termination of excitatory neurotransmission in central nervous system. Mutations are associated with episodic ataxia, Type 6. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2014]

Uniprot Description

SLC1A3: Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia. Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter; Mitochondrial; Transporter, SLC family; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 5p13

Cellular Component: cell surface; cell projection; membrane; cell soma; fibril; integral to membrane; plasma membrane

Molecular Function: glutamate binding; high-affinity glutamate transmembrane transporter activity; sodium:dicarboxylate symporter activity; L-glutamate transmembrane transporter activity

Biological Process: response to drug; response to light stimulus; cranial nerve development; auditory behavior; glutamate biosynthetic process; positive regulation of synaptic transmission; response to antibiotic; L-glutamate import; synaptic transmission; sensory perception of sound; neuron morphogenesis during differentiation; gamma-aminobutyric acid biosynthetic process; response to wounding; neurotransmitter uptake; ion transport; neuromuscular process controlling balance; transmembrane transport

Disease: Episodic Ataxia, Type 6