Human dsDNA IgG ELISA Kit | dsDNA elisa kit
dsDNA IgG ELISA
Reactivity
Human
Synonyms
dsDNA IgG; dsDNA IgG ELISA; dsDNA elisa kit
Reactivity
Human
Specificity
The solid phase is coated with recombinant double-stranded DNA (dsDNA). Due to a newly developed coating process the antigenic structure is conserved and during coating no sequences of single-stranded DNA occur. Therefore the Anti-dsDNA IgG test kit recognizes only autoantibodies specific for double-stranded DNA.
Samples
Human serum or plasma
Sensitivity
The lower detection limit for Anti-dsDNA IgG has been determined at 1.0 IU/ml.
Intended Uses
Anti-dsDNA is an indirect solid phase enzyme immunoassay (ELISA) for the quantitative measurement of IgG class autoantibodies against double-stranded DNA in human serum or plasma.
Preparation and Storage
2 - 8 degree C
Related Product Information for dsDNA elisa kit
Principle of the assay: Human recombinant double-stranded DNA (dsDNA) is bound to microwells. Antibodies to this antigen, if present in diluted serum, bind in the microwells. Washing of the microwells removes unbound serum antibodies. Horseradish peroxidase (HRP) conjugated anti-human IgG immunologically bind to the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color. The addition of an acid stops the reaction forming a yellow end-product. The intensity of this yellow color is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgG antibodies present in the original sample.
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Product Notes
The Human dsDNA (Catalog #AAA494727-ORG 604) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA494727-ORG 604 ELISA Kit recognizes Human dsDNA. It is sometimes possible for the material contained within the vial of "dsDNA IgG, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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