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Human MPO ELISA Kit | MPO elisa kit

Anti-MPO (p-ANCA) ELISA

Reactivity
Human
Synonyms
MPO; Anti-MPO (p-ANCA) ELISA; MPO elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
The microplate for Anti-MPO is coated with highly purified myeloperoxidase (MPO), isolated from human granulocytes showing high specific peroxidase activity. The test kit is specific only for autoantibodies directed to the respective antigen. No crossreactivities have been observed.
Sequence Length
745
Samples
Human serum or plasma
Sensitivity
The lower detection limit for Anti-MPO has been determined at 0.5 U/ml.
Intended Uses
Anti-MPO is an indirect solid phase enzyme immunoassay (ELISA) for the quantitative measurement of IgG class autoantibodies against myeloperoxidase (MPO) in human serum or plasma.
Preparation and Storage
2 - 8 degree C
Related Product Information for MPO elisa kit
Principle of the assay: Highly purified myeloperoxidase (MPO) is bound to microwells. Antibodies to this antigen, if present in diluted serum, bind in the microwells. Washing of the microwells removes unbound serum antibodies. Horseradish peroxidase (HRP) conjugated anti-human IgG immunologically bind to the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color. The addition of an acid stops the reaction forming a yellow end-product. The intensity of this yellow color is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgG antibodies present in the original sample.
Product Categories/Family for MPO elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
87,248 Da
NCBI Official Full Name
myeloperoxidase
NCBI Official Synonym Full Names
myeloperoxidase
NCBI Official Symbol
MPO
NCBI Protein Information
myeloperoxidase
UniProt Protein Name
Myeloperoxidase
Protein Family
UniProt Gene Name
MPO
UniProt Synonym Gene Names
MPO
UniProt Entry Name
PERM_HUMAN

NCBI Description

Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014]

Uniprot Description

MPO: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. Homodimer; disulfide-linked. Each monomer consists of a light and a heavy chain. Belongs to the peroxidase family. XPO subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; EC 1.11.2.2

Chromosomal Location of Human Ortholog: 17q23.1

Cellular Component: extracellular space; azurophil granule; mitochondrion; lysosome; nucleus; secretory granule

Molecular Function: heparin binding; peroxidase activity; metal ion binding; heme binding; chromatin binding

Biological Process: response to food; respiratory burst during defense response; removal of superoxide radicals; response to mechanical stimulus; hydrogen peroxide catabolic process; response to lipopolysaccharide; response to yeast; defense response; response to oxidative stress; defense response to fungus; negative regulation of apoptosis; aging

Disease: Myeloperoxidase Deficiency; Alzheimer Disease

Research Articles on MPO

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Product Notes

The Human MPO mpo (Catalog #AAA494683-ORG 519) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA494683-ORG 519 ELISA Kit recognizes Human MPO. It is sometimes possible for the material contained within the vial of "MPO, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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