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Human Complement C4a des Arg ELISA Kit

Complement C4a des Arg (Human) ELISA

Reactivity
Human
Synonyms
Complement C4a des Arg; Complement C4a des Arg (Human) ELISA; Complement C4a des Arg elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Samples
Plasma (EDTA/Futhan)
Assay Type
Competitive
Detection Range
0 / 0.78 - 200 ng/mL
Sensitivity
0.76 ng/mL
Standards
6 standards, serially diluted from 1 ready to use standard.
Sample Volume
100 uL of properly prepared/diluted unknown / determination.
Design
Competitive enzyme immunoassay (ELISA).
Description Protocol
MyBioSource human Complement C4a des Arg kit is a competitive immunoassay for the determination of human C4a des Arg in plasma. Please read the complete kit insert before performing this assay. The kit uses a polyclonal antibody to human C4a des Arg to bind, in a competitive manner, the human C4a des Arg in the standard or sample or an alkaline phosphatase molecule which has human C4a des Arg attached to it. After incubation at room temperature the excess reagents are washed away and substrate is added. After a short incubation, the enzyme reaction is stopped and the yellow color generated is read on a microplate reader at 405nm. The intensity of the bound yellow color is inversely proportional to the concentration of human C4a des Arg in either standards or samples. The measured optical density is used to calculate the concentration of human C4a des Arg. For further
Assay Description
2 hour incubation (RT) + 1 hour (37 degree C) = 3 hours total incubation time.
Preparation and Storage
2 - 8 degree C
Related Product Information for Complement C4a des Arg elisa kit
Competitive enzyme immunoassay (ELISA) for the determination of human C4a desArg in plasma. For research use only.
Product Categories/Family for Complement C4a des Arg elisa kit

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Product Notes

The Human Complement C4a des Arg (Catalog #AAA494468) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA494468 ELISA Kit recognizes Human Complement C4a des Arg. It is sometimes possible for the material contained within the vial of "Complement C4a des Arg, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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