Human Complement C3a des Arg ELISA Kit
Complement C3a des Arg (Human) ELISA
Reactivity
Human
Synonyms
Complement C3a des Arg; Complement C3a des Arg (Human) ELISA; Complement C3a des Arg elisa kit
Reactivity
Human
Samples
Human C3a des Arg plasma in EDTA/Futhan tubes
Assay Type
Competitive
Detection Range
0 / 0.313 - 20.0 ng/mL
Sensitivity
0.120 ng/mL
Standards
7 calibrators, serially diluted from 1 prepared lyophilized calibrator.
Sample Volume
100 uL of properly prepared/diluted unknown / determination.
Design
Enzyme immunoassay (ELISA) technique.
Description Protocol
The Complement C3a des Arg (human), EIA kit is a competitive immunoassay for the determination of C3a des Arg in plasma. Please read the complete kit insert before performing this assay. The kit uses a polyclonal antibody to human C3a des Arg to bind, in a competitive manner, the human C3a des Arg in the sample or an alkaline phosphatase molecule which has human C3a des Arg attached to it. After incubation at room temperature the excess reagents are washed away and substrate is added. After a short incubation, the enzyme reaction is stopped and the yellow color generated is read on a microplate reader at 405nm. The intensity of the bound yellow color is inversely proportional to the concentration of human C3a des Arg in either standards or samples. The measured optical density is used to calculate the concentration of human C3a des Arg. For further explanation of the principles and practice of immunoassays please see the excellent books by Chard1 or Tijssen2.
Assay Description
1 hour incubation (RT) + 2 hours (RT) + 1 hour (37 degree C) = 4 hours total incubation time.
Preparation and Storage
All components of this kit, except the conjugate and lyophilized standard, are stable at 4 degree C until the kit's expiration date.The human C3a des Arg conjugate and lyophilized standard should be stored at -20 degree C upon receipt.
Related Product Information for Complement C3a des Arg elisa kit
Competitive immunoassay (ELISA) for the determination of C3a des Arg in plasma. For research use only.
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Product Notes
The Human Complement C3a des Arg (Catalog #AAA494467) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA494467 ELISA Kit recognizes Human Complement C3a des Arg. It is sometimes possible for the material contained within the vial of "Complement C3a des Arg, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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