Human Helicobacter pylori IgA ELISA Kit | IgA elisa kit
Helicobacter pylori IgA ELISA
Reactivity
Human
Synonyms
Helicobacter pylori IgA; Helicobacter pylori IgA ELISA; IgA elisa kit
Reactivity
Human
Specificity
The Bordetella pertussis IgA, IgG, and IgM ELISA was verified in an internal study. The sensitivity and specificity in both cases exceeded 96%.
Application Notes
1 hour (37 degree C) + 30 min. (37 degree C) + 30 min. (37 degree C) = 2 hours total incubation time.
Samples
Serum And Plasma
Preparation and Storage
Store at 2-8 degree C
Related Product Information for IgA elisa kit
Intended Uses: The Helicobacter pylori IgA Antibody ELISA Test Kit has been designed for the determination of specific IgA antibodies against Helicobacter pylori in serum and plasma. For research use only.
Principle of the Assay: The MBS Helicobacter pylori IgA antibody test kit is based on the principle of the enzyme immunoassay (EIA). Helicobacter antigen is bound on the surface of the microtiter strips. Diluted unknowns are pipetted into the wells of the microtiter plate. A binding between the IgA antibodies of the serum and the immobilized Helicobacter antigen takes place. After a one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use anti-human-IgA peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm.
Principle of the Assay: The MBS Helicobacter pylori IgA antibody test kit is based on the principle of the enzyme immunoassay (EIA). Helicobacter antigen is bound on the surface of the microtiter strips. Diluted unknowns are pipetted into the wells of the microtiter plate. A binding between the IgA antibodies of the serum and the immobilized Helicobacter antigen takes place. After a one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use anti-human-IgA peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm.
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Product Notes
The Human IgA (Catalog #AAA490273) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA490273 ELISA Kit recognizes Human IgA. 1 hour (37 degree C) + 30 min. (37 degree C) + 30 min. (37 degree C) = 2 hours total incubation time. Researchers should empirically determine the suitability of the IgA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Helicobacter pylori IgA, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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