Thrombin IgG/IgM ELISA Kit
Thrombin IgG/IgM ELISA
Synonyms
Thrombin IgG/IgM; Thrombin IgG/IgM ELISA; Thrombin IgG/IgM elisa kit
Specificity
The microplate is coated with native human thrombin. No cross reactivities to other autoantigens have been found.
Application Notes
30 min. incubation (RT) + 30 min. (RT) + 30 min. (RT) + 5 min. (RT) = 1 hour, 35 min. total incubation time.
Samples
Human serum
Sensitivity
1.0 U/ml
Preparation and Storage
Store at 2-8 degree C
Related Product Information for Thrombin IgG/IgM elisa kit
Intended Uses: This Thrombin IgG/M ELISA is a solid phase enzyme immunoassay employing native human thrombin for the quantitative and qualitative detection of IgG and/or IgM antibodies against thrombin in human serum.
Principle of the Assay: Samples diluted 1:101 are incubated in the microplates coated with the specific antigen. The antibodies, if present in the specimen, bind to the antigen. The unbound fraction is washed off in the following step. Afterwards anti-human immunoglobulins conjugated to horseradish peroxidase (conjugate) are incubated and react with the antigen-antibody complex of the samples in the microplates. Unbound conjugate is washed off in the following step. Addition of TMBsubstrate generates an enzymatic colorimetric (blue) reaction, which is stopped by diluted acid (color changes to yellow). The intensity of color formation from the chromogen is a function of the amount of conjugate bound to the antigen-antibody complex and this is proportional to the initial concentration of the respective antibodies in the sample.
Background: Thrombin is not a normal constituent of the circulating blood. It is generated by the catalytic cleavage of its plasma precursor, prothrombin (factor II), by the activated Stuart factor (factor Xa). This is the final step of the intrinsic and extrinsic pathways of coagulation. The transformation requires the presence of an activated cofactor, factor Va, released from factor V by thrombin itself, and whose binding to prothrombin accelerates the activity of factor Xa in a non-enzymatic manner. Thrombin is a glycoprotein formed by two peptides chains of 36 and 259 amino-acids linked by disulfure bonds. Three important sites have been identified on the surface of the enzyme: The catalytic site that confers to the molecule its serine protease activity, the exosite one responsible for the binding of the substrate (fibrinogen or thrombin receptor) and the exosite two responsible for the binding of antithrombin III and inactivation of thrombin. Thrombin is, however, more than a simple plasma enzyme. Its properties to stimulate platelets and cause them to expand aggregate and release components of the alpha and dense granules were recognized earlier on.
Principle of the Assay: Samples diluted 1:101 are incubated in the microplates coated with the specific antigen. The antibodies, if present in the specimen, bind to the antigen. The unbound fraction is washed off in the following step. Afterwards anti-human immunoglobulins conjugated to horseradish peroxidase (conjugate) are incubated and react with the antigen-antibody complex of the samples in the microplates. Unbound conjugate is washed off in the following step. Addition of TMBsubstrate generates an enzymatic colorimetric (blue) reaction, which is stopped by diluted acid (color changes to yellow). The intensity of color formation from the chromogen is a function of the amount of conjugate bound to the antigen-antibody complex and this is proportional to the initial concentration of the respective antibodies in the sample.
Background: Thrombin is not a normal constituent of the circulating blood. It is generated by the catalytic cleavage of its plasma precursor, prothrombin (factor II), by the activated Stuart factor (factor Xa). This is the final step of the intrinsic and extrinsic pathways of coagulation. The transformation requires the presence of an activated cofactor, factor Va, released from factor V by thrombin itself, and whose binding to prothrombin accelerates the activity of factor Xa in a non-enzymatic manner. Thrombin is a glycoprotein formed by two peptides chains of 36 and 259 amino-acids linked by disulfure bonds. Three important sites have been identified on the surface of the enzyme: The catalytic site that confers to the molecule its serine protease activity, the exosite one responsible for the binding of the substrate (fibrinogen or thrombin receptor) and the exosite two responsible for the binding of antithrombin III and inactivation of thrombin. Thrombin is, however, more than a simple plasma enzyme. Its properties to stimulate platelets and cause them to expand aggregate and release components of the alpha and dense granules were recognized earlier on.
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Product Notes
The Thrombin IgG/IgM (Catalog #AAA490226) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. 30 min. incubation (RT) + 30 min. (RT) + 30 min. (RT) + 5 min. (RT) = 1 hour, 35 min. total incubation time. Researchers should empirically determine the suitability of the Thrombin IgG/IgM for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Thrombin IgG/IgM, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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