Flow Cytometry (FC/FACS)
(Flow cytometry using the Anti-IFN-gamma receptor 1 antibody GR20 (MBS488632). Paraformaldehyde fixed mouse splenocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (MBS488149; isotype control, black line) or the rabbit IgG version of GR20 (, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Rabbit anti-Mouse IFN-gamma receptor 1 Monoclonal Antibody | anti-IFN-gamma antibody
Anti-IFN-gamma receptor 1 [GR20]
Gene Names
Ifngr1; Ifgr; CD119; Ifngr; Nktar; IFN-gammaR
Reactivity
Mouse
Applications
Neutralization Assay, Flow Cytometry, Functional Assay
Purity
Purified antibody. Protein A affinity purified
Synonyms
IFN-gamma receptor 1; Monoclonal Antibody; Anti-IFN-gamma receptor 1 [GR20]; CD119; Interferon gamma receptor 1; IFN gamma receptor 1; IFN-gamma-R1; Interferon gamma receptor alpha-chain; IFN-gamma-R-alpha; anti-IFN-gamma antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG, kappa
Clone Number
GR20
Specificity
This antibody recognizes IFN-gammaRalpha, which is a class II cytokine receptor family member that serves as a IFN-gamma-binding chain associated with the IFN-gamma beta.
Purity/Purification
Purified antibody. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-IFN-gamma antibody
Blocking (BL), Neutralizing (NEU), Flow Cytometry (FC/FACS)
Application Notes
This antibody is recomended as an efficient blocking agent for the interferon gamma receptor (Basu et al. 1988). It has been also used in the flow cytometric analyses of this receptor protein.
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
PMID
2970637
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Flow Cytometry (FC/FACS)
(Flow cytometry using the Anti-IFN-gamma receptor 1 antibody GR20 (MBS488632). Paraformaldehyde fixed mouse splenocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (MBS488149; isotype control, black line) or the rabbit IgG version of GR20 (, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Flow Cytometry (FC/FACS)
(Flow cytometry using the Anti-IFN-gamma receptor 1 antibody GR20 (MBS488632). Paraformaldehyde fixed mouse splenocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (MBS488149; isotype control, black line) or the rabbit IgG version of GR20 (, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Related Product Information for anti-IFN-gamma antibody
Recombinant monoclonal antibody to IFN-gamma receptor 1. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma GR20.
This antibody was raised by immunising rats with interferon gamma receptor.
This antibody was raised by immunising rats with interferon gamma receptor.
Product Categories/Family for anti-IFN-gamma antibody
References
Basu et al. Purification and partial characterization of a receptor protein for mouse interferon gamma. Proc Natl Acad Sci U S A. 1988 Sep;85(17):6282-6.
Note on publication: This article describes the generation and characterization of the GR-20 antibody.
Note on publication: This article describes the generation and characterization of the GR-20 antibody.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52,343 Da
NCBI Official Full Name
interferon gamma receptor 1
NCBI Official Synonym Full Names
interferon gamma receptor 1
NCBI Official Symbol
Ifngr1
NCBI Official Synonym Symbols
Ifgr; CD119; Ifngr; Nktar; IFN-gammaR
NCBI Protein Information
interferon gamma receptor 1; IFN-gamma-R1; INF-g receptor; IFN-gamma receptor 1; interferon-gamma receptor 1
UniProt Protein Name
Interferon gamma receptor 1
UniProt Gene Name
Ifngr1
UniProt Synonym Gene Names
Ifngr; IFN-gamma receptor 1
UniProt Entry Name
INGR1_MOUSE
Uniprot Description
Function: Receptor for interferon gamma. Two receptors bind one interferon gamma dimer.
Subcellular location: Membrane; Single-pass type I membrane protein.
Post-translational modification: Phosphorylated at Ser/Thr residues.
Sequence similarities: Belongs to the type II cytokine receptor family.Contains 2 fibronectin type-III domains.Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Sequence caution: The sequence AAA37895.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
Research Articles on IFN-gamma
Similar Products
Product Notes
The IFN-gamma ifngr1 (Catalog #AAA488632) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-IFN-gamma receptor 1 [GR20] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IFN-gamma receptor 1 can be used in a range of immunoassay formats including, but not limited to, Blocking (BL), Neutralizing (NEU), Flow Cytometry (FC/FACS). This antibody is recomended as an efficient blocking agent for the interferon gamma receptor (Basu et al. 1988). It has been also used in the flow cytometric analyses of this receptor protein. Researchers should empirically determine the suitability of the IFN-gamma ifngr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IFN-gamma receptor 1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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