Lymphotoxin alpha Recombinant Antibody | LTalpha recombinant antibody
Anti-Lymphotoxin alpha [3F12.2D3]
Gene Names
LTA; LT; TNFB; TNFSF1; TNLG1E
Reactivity
Human
Applications
Neutralization Assay, ELISA, Flow Cytometry, Functional Assay
Purity
Protein A Affinity Purified
Synonyms
Lymphotoxin alpha; Monoclonal Recombinant Antibody; Anti-Lymphotoxin alpha [3F12.2D3]; chimeric 3F12; LT; LTalpha3; Tumor necrosis factor beta; Tumor necrosis factor-beta; tumor necrosis factor-beta; TNF beta; TNF-beta; Lymphotoxin-alpha; LTalpha recombinant antibody
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG2b, Kappa
Clone Number
3F12.2D3
Specificity
3F12 binds to human LTalpha3 and LTalphabeta (with a KD of ~0.3 nM, determined by BIACORE; ~ 37 pM, determined by ELISA) in both its soluble homotrimeric and membrane heterotrimeric forms. LTalpha belongs to the tumor necrosis factor (TNF) superfamily and is secreted as a homo-trimer (LTalpha3), or is expressed on the cell surface in complex with LTbeta. Lymphotoxin is produced by lymphocytes and in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. LTalpha3 signaling induces target cells to upregulate many chemokines and cytokines in an NFkB-dependent manner.
Purity/Purification
Protein A Affinity Purified
Concentration
1 mg/ml (varies by lot)
Sequence Length
205
Applicable Applications for LTalpha recombinant antibody
Neutralization, ELISA (EIA), Flow Cytometry (FC/FACS), Surface Plasmon Resonance (SPR)
Application Notes
The properties of the chimieric 3F12 is equivalent to that of the original 3F12 mAb. 3F12 is able to bind to Th2 cells and additionally is can functionally block human LTalpha3 versus an isotype control (trastuzumab)-LTalpha3-induced ICAM upregulation is blocked on HUVEC (Human Umbilical Endothelial Cells) (IC50 0.04 nM) and LTalpha3-induced IL-8 is blocked in A549 cells. The antibody has also been used in ELISA studies to detect LT-alpha and to measure binding.
Immunogen
3F12 was prepared by hyperimmunizing BALB/c mice with purified recombinant human LTalpha expressed in E. coli-the hybridoma clone was selected for its ability to bind LTalpha3 by ELISA. The CDRs of the antibody were grafted to the framework regions of the mice mAb 2G7 to create the chimeric version of the antibody.
Buffer Composition
PBS only with 0.02% Proclin 300.
Labeled Substance
None
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Related Product Information for LTalpha recombinant antibody
Recombinant monoclonal antibody to Lymphotoxin alpha. Manufactured using a proprietary Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 3F12.2D3.
3F12 was prepared by hyperimmunizing BALB/c mice with purified recombinant human LTalpha expressed in E. coli-the hybridoma clone was selected for its ability to bind LTalpha3 by ELISA. The CDRs of the antibody were grafted to the framework regions of the mice mAb 2G7 to create the chimeric version of the antibody.
3F12 was prepared by hyperimmunizing BALB/c mice with purified recombinant human LTalpha expressed in E. coli-the hybridoma clone was selected for its ability to bind LTalpha3 by ELISA. The CDRs of the antibody were grafted to the framework regions of the mice mAb 2G7 to create the chimeric version of the antibody.
Product Categories/Family for LTalpha recombinant antibody
References
Catellana et al. Resurrection of a Clinical Antibody: Template ProteoGenomic de novoProteomic Sequencing and Reverse Engineering of an Anti-Lymphotoxin Alpha Antibody Proteomics. 2011 Feb; 11(3): 395-405. PMID:21268269
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,297 Da
NCBI Official Full Name
lymphotoxin-alpha
NCBI Official Synonym Full Names
lymphotoxin alpha
NCBI Official Symbol
LTA
NCBI Official Synonym Symbols
LT; TNFB; TNFSF1; TNLG1E
NCBI Protein Information
lymphotoxin-alpha
UniProt Protein Name
Lymphotoxin-alpha
UniProt Gene Name
LTA
UniProt Synonym Gene Names
TNFB; TNFSF1; LT-alpha
NCBI Description
The encoded protein, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4, myocardial infarction, non-Hodgkin's lymphoma, and psoriatic arthritis. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jul 2012]
Uniprot Description
Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo.
Research Articles on LTalpha
Similar Products
Product Notes
The LTalpha lta (Catalog #AAA488356) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Lymphotoxin alpha [3F12.2D3] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Lymphotoxin alpha can be used in a range of immunoassay formats including, but not limited to, Neutralization, ELISA (EIA), Flow Cytometry (FC/FACS), Surface Plasmon Resonance (SPR). The properties of the chimieric 3F12 is equivalent to that of the original 3F12 mAb. 3F12 is able to bind to Th2 cells and additionally is can functionally block human LTalpha3 versus an isotype control (trastuzumab)-LTalpha3-induced ICAM upregulation is blocked on HUVEC (Human Umbilical Endothelial Cells) (IC50 0.04 nM) and LTalpha3-induced IL-8 is blocked in A549 cells. The antibody has also been used in ELISA studies to detect LT-alpha and to measure binding. Researchers should empirically determine the suitability of the LTalpha lta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lymphotoxin alpha, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
