His tag Recombinant Antibody
Anti-His tag (C-term) [3D5]
Applications
Western Blot, ELISA
Purity
Protein A Affinity Purified
Synonyms
His tag; Anti-His tag (C-term) [3D5]; polyhistidine tag; His tag recombinant antibody
Isotype
Rat IgG2c, kappa
Clone Number
3D5
Specificity
Carboxy-terminal oligo-histidine tags (6x His tags) of fusion proteins.
Purity/Purification
Protein A Affinity Purified
Applicable Applications for His tag recombinant antibody
Western Blot (WB), ELISA (EIA)
Buffer Composition
PBS only with 0.02% Proclin 300.
Immunogen
Purified his tagged Fab fragment.
Chimeric Use Statement
This is a chimeric rat antibody, based on the original mouse IgG2b format, created for improved compatibility with existing reagents, assays and techniques.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Related Product Information for His tag recombinant antibody
Recombinant monoclonal antibody to His tag (C-term). Manufactured using a Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 3D5. This is a chimeric rat antibody, based on the original mouse IgG2b format, created for improved compatibility with existing reagents, assays and techniques.
Product Categories/Family for His tag recombinant antibody
References
Lindner P, Bauer K, Krebber A, Nieba L, Kremmer E, Krebber C, Honegger A, Klinger B, Mocikat R, Plckthun A. Specific detection of his-tagged proteins with recombinant anti-His tag scFv-phosphatase or scFv-phage fusions. Biotechniques. 1997 Jan; 22(1):140-9. PMID:8994661
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Product Notes
The His tag (Catalog #AAA488031) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's His tag can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). Researchers should empirically determine the suitability of the His tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "His tag, Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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