M-CSF active protein

Recombinant M-CSF

Cat. Num. AAA48318

• Gene Names: Csf1; op; Csfm; MCSF; C87615
• Applications: SDS-Page
• Purity: > 90% by SDS - PAGE
• Synonyms: M-CSF; N/A; Recombinant M-CSF; M-Csf; Macrophage colony-stimulating factor 1; CSF-1; MCSF; Csf1; C87615; op; Processedmacrophage colony-stimulating factor 1; M-CSF active protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS - PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
• Concentration: 1mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 33-187aa
• Sequence: KEVSEHC SHMIGNGHLK VLQQLIDSQM ETSCQIAFEF VDQEQLDDPV CYLKKAFFLV QDIIDETMRF KDNTPNANAT ERLQELSNNL NSCFTKDYEE QNKACVRTFH ETPLQLLEKI KNFFNETKNL LEKDWNIFTK NCNNSFAKCS SRDVVTKP
• Sequence Length: 257

• Applicable Applications for M-CSF active protein: Bioactivity, SDS-PAGE
• Tag: His-Tag
• Biological Activity: Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 4 ng/ml.
• Predicted nterm: Ala
• Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
• Preparation and Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoidrepeated freezing and thawing cycles.

Bioactivity

(Mouse M-CSF stimulates cell proliferation of the M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 range is )

SDS-PAGE

(3ug by SDS-PAGE under reducing condition andvisualized by coomassie blue stain)

Related Product Information for M-CSF active protein

M-CSF, also known as macrophage colony-stimulating factor 1 isoform 1, is one of the hematopoietic growth factors that regulate the growth and differentiation of blood cells. This protein is produced by monocytes,granulocytes, endothelial cells, and fibroblasts. It stimulates the formation of macrophage colonies, enhancesantibody-dependent, cell-mediated cytotoxicity by monocytes and macrophages, and inhibits bone resorption byosteoclasts. It plays an essential role in the regulation of survival, proliferation and differentiation ofhematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. This proteinpromotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and ininflammatory processes. Recombinant mouse M-CSF, fused to His-tag at C-terminus, was expressed in insect celland purified by using conventional chromatography techniques.

Product Categories/Family for M-CSF active protein

Immunology

References

Richardsen E., et al. (2018) Anticancer Res. 35(2):865-874.;;Braza MS., et al. (2018) Am J Transplant. 18:1247-1255.

NCBI and Uniprot Product Information

• NCBI GI #: 166064046
• NCBI GeneID: 12977
• NCBI Accession #: NP_001107001.1
• NCBI GenBank Nucleotide #: NP_001107001.1
• UniProt Accession #: P07141
• Molecular Weight: 19.1kDa (164aa)
• NCBI Official Full Name: macrophage colony-stimulating factor 1 isoform 2
• NCBI Official Synonym Full Names: colony stimulating factor 1 (macrophage)
• NCBI Official Symbol: Csf1
• NCBI Official Synonym Symbols: op; Csfm; MCSF; C87615
• NCBI Protein Information: macrophage colony-stimulating factor 1
• UniProt Protein Name: Macrophage colony-stimulating factor 1
• UniProt Gene Name: Csf1
• UniProt Synonym Gene Names: Csfm; CSF-1; MCSF
• UniProt Entry Name: CSF1_MOUSE