Western Blot (WB)
(Western blot analysis using WT1 mAb against human WT1 (AA:314-479) recombinant protein. (Expected MW is 47.6 kDa))
Mouse anti-Human WT1 Monoclonal Antibody | anti-WT1 antibody
Anti-WT1 Mouse mAb
ELISA: 1/10000
Store at -20°C long term.
Avoid freeze/thaw cycle.
Western Blot (WB)
(Western blot analysis using WT1 mAb against human WT1 (AA:314-479) recombinant protein. (Expected MW is 47.6 kDa))
Western Blot (WB)
(Western blot analysis using WT1 mAb against human WT1 (AA:314-479) recombinant protein. (Expected MW is 47.6 kDa))
Western Blot (WB)
(Western blot analysis using WT1 mAb against HEK293 (1) and WT1 (AA)
Western Blot (WB)
(Western blot analysis using WT1 mAb against HEK293 (1) and WT1 (AA)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Hela cells using WT1 mouse mAb (green) and negative control (red).)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Hela cells using WT1 mouse mAb (green) and negative control (red).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Mar 2015]
Uniprot Description
Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (PubMed:7862533, PubMed:17716689, PubMed:25258363). Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors (PubMed:15520190). Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed:16934801). Isoform 1 has lower affinity for DNA, and can bind RNA (PubMed:19123921).
Research Articles on WT1
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Product Notes
The WT1 wt1 (Catalog #AAA476771) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-WT1 Mouse mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's WT1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC). FC: 1/200 - 1/400 ELISA: 1/10000. Researchers should empirically determine the suitability of the WT1 wt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WT1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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