Western Blot (WB)
(Western blot detection of GRK2 in C6, Jurkat, K562 and 3T3 cell lysates using GRK2 mouse mAb (1:1000 diluted).Predicted band size:80KDa.Observed band size:80KDa.)
Mouse GRK2 Monoclonal Antibody | anti-GRK2 antibody
Anti-GRK2 Mouse mAb
Western Blot (WB)
(Western blot detection of GRK2 in C6, Jurkat, K562 and 3T3 cell lysates using GRK2 mouse mAb (1:1000 diluted).Predicted band size:80KDa.Observed band size:80KDa.)
Western Blot (WB)
(Western blot detection of GRK2 in C6, Jurkat, K562 and 3T3 cell lysates using GRK2 mouse mAb (1:1000 diluted).Predicted band size:80KDa.Observed band size:80KDa.)
Immunofluorescence (IF)
(Immunofluorescence analysis of NIH/3T3 cells using GRK2 mouse mAb (green). Blue)
Immunofluorescence (IF)
(Immunofluorescence analysis of NIH/3T3 cells using GRK2 mouse mAb (green). Blue)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues (left) and cervical cancer tissues (right) using GRK2 mouse mAb with DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues (left) and cervical cancer tissues (right) using GRK2 mouse mAb with DAB staining.)
UniPort Summary Function: Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner.
NCBI and Uniprot Product Information
NCBI Description
The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq, Jul 2008]
Uniprot Description
GRK2: a ubiquitous protein kinase of the GRK family. Phosphorylates the beta-2-adrenergic receptor and related G-protein-coupled receptors. Appears to mediate agonist-specific desensitization observed at high agonist concentrations. Expression level is consistently elevated in chronic human heart failure. Mouse models of severe heart failure have been used to demonstrate that inhibition of BARK1 with a peptide inhibitor is sufficient to increase mean survival, reduce dialation and improve cardiac function.
Protein type: AGC group; BARK subfamily; EC 2.7.11.15; GRK family; Kinase, protein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor)
Chromosomal Location of Human Ortholog: 11q13.2
Cellular Component: cytoplasm; cytosol; membrane; plasma membrane
Molecular Function: alpha-2A adrenergic receptor binding; beta-adrenergic receptor kinase activity; Edg-2 lysophosphatidic acid receptor binding; G-protein coupled receptor kinase activity; protein binding; protein kinase activity
Biological Process: acetylcholine receptor signaling, muscarinic pathway; cardiac muscle contraction; desensitization of G-protein coupled receptor protein signaling pathway; entry of virus into host cell; negative regulation of striated muscle contraction; negative regulation of the force of heart contraction by chemical signal; peptidyl-serine phosphorylation; positive regulation of catecholamine secretion; receptor internalization; tachykinin signaling pathway; viral genome replication
Research Articles on GRK2
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Product Notes
The GRK2 grk2 (Catalog #AAA476158) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-GRK2 Mouse mAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GRK2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the GRK2 grk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GRK2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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