Mouse anti-Human CD276 Monoclonal Antibody | anti-CD276 antibody

Anti-CD276 Mouse mAb

Cat. Num. AAA476069

• Gene Names: CD276; B7H3; B7-H3; B7RP-2; 4Ig-B7-H3
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Synonyms: CD276; Monoclonal Antibody; Anti-CD276 Mouse mAb; B7H3; B7-H3; 4Ig-B7-H3; anti-CD276 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 6A1
• Form/Format: Ascitic fluid containing 0.03% sodium azide.
• Sequence Length: 534

• Applicable Applications for anti-CD276 antibody: Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Immunogen: Purified recombinant fragment of human CD276 expressed in E Coli.

Western Blot (WB)

(Western blot analysis using CD276 mAb against HEK293 (1) and CD276 (AA)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of PC-3 cells using CD276 mouse mAb (green) and negative control (purple).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded cervical cancer tissues (left) and ovarian cancer tissues (right) using CD276 mouse mAb with DAB staining.)

Related Product Information for anti-CD276 antibody

Entrez Summary: Costimulatory B7 molecules (e.g., B7-1, or CD80; MIM 112203) signal through CD28 (MIM 186760) family molecules such as CD28, CTLA4 (MIM 123890), and ICOS (MIM 604558). May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.

NCBI and Uniprot Product Information

• NCBI GI #: 67188443
• NCBI GeneID: 80381
• NCBI Accession #: NP_001019907.1
• NCBI GenBank Nucleotide #: NM_001024736.1
• UniProt Accession #: Q5ZPR3; Q6P5Y4; Q6UXI2; Q8NBI8; Q8NC34; Q8NCB6; Q9BXR1
• Molecular Weight: 57
• NCBI Official Full Name: CD276 antigen isoform a
• NCBI Official Synonym Full Names: CD276 molecule
• NCBI Official Symbol: CD276
• NCBI Official Synonym Symbols: B7H3; B7-H3; B7RP-2; 4Ig-B7-H3
• NCBI Protein Information: CD276 antigen
• UniProt Protein Name: CD276 antigen
• Protein Family: CD276 antigen
• UniProt Gene Name: CD276
• UniProt Synonym Gene Names: B7H3; B7-H3

NCBI Description

The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

CD276: May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling. Belongs to the immunoglobulin superfamily. BTN/MOG family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 15q24.1

Molecular Function: protein binding

Biological Process: positive regulation of interferon-gamma biosynthetic process; positive regulation of T cell proliferation; T cell activation