Rabbit anti-Human, Mouse Ephrin B3 Polyclonal Antibody | anti-EFNB3 antibody

Ephrin B3 Rabbit pAb

Cat. Num. AAA4758630

• Gene Names: EFNB3; EFL6; EPLG8; LERK8
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: Ephrin B3; Polyclonal Antibody; Ephrin B3 Rabbit pAb; EFL6; EPLG8; LERK8; anti-EFNB3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: Affinity purified
• Form/Format: -20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

• Applicable Applications for anti-EFNB3 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:200-1:1000
• Immunogen: Synthetic peptide of human EFNB3
• Conjugation: Unconjugated
• Molecular Function: EFNB3, a member of the ephrin gene family, is important in brain development as well as in its maintenance. Moreover, since levels of EFNB3 expression were particularly high in several forebrain subregions compared to other brain subregions, it may play a pivotal role in forebrain function. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. EPH Receptors typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin ligands and receptors have been named by the Eph Nomenclature Committee (1997). Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are similarly divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.
• Modification: Unmodified
• Cell Position: Membrane
• Preparation and Storage: Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: NIH/3T3 and SKOV3 cell lysates, Primary antibody: 862965(EFNB3 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 second)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 862965(EFNB3 Antibody) at dilution 1/25. (Original magnification: ×200))

Product Categories/Family for anti-EFNB3 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 4503489
• NCBI GeneID: 1949
• NCBI Accession #: NP_001397
• NCBI GenBank Nucleotide #: NM_001406.3
• UniProt Accession #: Q15768; O00680; Q8TBH7; Q92875; B2RBW2; D3DTQ6
• Molecular Weight: 24.6 kDa (224aa)
• NCBI Official Full Name: ephrin-B3
• NCBI Official Synonym Full Names: ephrin B3
• NCBI Official Symbol: EFNB3
• NCBI Official Synonym Symbols: EFL6; EPLG8; LERK8
• NCBI Protein Information: ephrin-B3
• UniProt Protein Name: Ephrin-B3
• Protein Family: Ephrin
• UniProt Gene Name: EFNB3
• UniProt Synonym Gene Names: EPLG8; LERK8; LERK-8
• UniProt Entry Name: EFNB3_HUMAN

NCBI Description

EFNB3, a member of the ephrin gene family, is important in brain development as well as in its maintenance. Moreover, since levels of EFNB3 expression were particularly high in several forebrain subregions compared to other brain subregions, it may play a pivotal role in forebrain function. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. EPH Receptors typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin ligands and receptors have been named by the Eph Nomenclature Committee (1997). Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are similarly divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. [provided by RefSeq, Jul 2008]

Uniprot Description

EFNB3: Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. May play a pivotal role in forebrain function. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons. Belongs to the ephrin family.

Protein type: Cell development/differentiation; Membrane protein, integral; Ligand, receptor tyrosine kinase

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: transmembrane-ephrin receptor activity; ephrin receptor binding

Biological Process: axon guidance; nervous system development; viral reproduction; cell-cell signaling; axon choice point recognition; ephrin receptor signaling pathway; adult walking behavior