Western Blot (WB)
(Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane 1-4: HEPG2, Hela, K562 and PC-3 cell lysates, Primary antibody: 824697(CYB5R1 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds)
Rabbit anti-Human CYB5R1 Polyclonal Antibody | anti-CYB5R1 antibody
CYB5R1 Rabbit pAb
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
CYB5R1; Polyclonal Antibody; CYB5R1 Rabbit pAb; B5R1; B5R2; B5R.1; NQO3A2; humb5R2; anti-CYB5R1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Affinity purified
Form/Format
-20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Applicable Applications for anti-CYB5R1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:200-1:1000
Immunogen
Fusion protein of human CYB5R1
Conjugation
Unconjugated
Molecular Function
NADH-cytochrome b5 reductases participate in a variety of physiological processes including biosynthesis of cholesterol, methemoglobin reduction of erythrocytes, elongation of fatty acids and metabolism of drugs. CYB5R1 (cytochrome b5 reductase 1), also known as NADH-cytochrome b5 reductase 1, B5R1, NQO3A2, humb5R2 or NAD(P)H:quinone oxidoreductase type 3 polypeptide A2, is a 305 amino acid single-pass membrane protein that contains one FAD-binding FR-type domain and belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Widely expressed, CYB5R1 binds FAD as a cofactor and is encoded by a gene located on human chromosome 1. Human chromosome 1 spans 260 million base pairs, contains over 3, 000 genes, comprises nearly 8% of the human genome and houses a large number of disease-associated genes, including those that are involved in familial adenomatous polyposis, Stickler syndrome, Parkinson's disease, Gaucher disease, schizophrenia and Usher syndrome.
Modification
Unmodified
Cell Position
Membrane
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.
Western Blot (WB)
(Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane 1-4: HEPG2, Hela, K562 and PC-3 cell lysates, Primary antibody: 824697(CYB5R1 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds)
Western Blot (WB)
(Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane 1-4: HEPG2, Hela, K562 and PC-3 cell lysates, Primary antibody: 824697(CYB5R1 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds)
Immunohistochemistry (IHC)
(The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 824697(CYB5R1 Antibody) at dilution 1/30. (Original magnification: ×200))
Immunohistochemistry (IHC)
(The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 824697(CYB5R1 Antibody) at dilution 1/30. (Original magnification: ×200))
Product Categories/Family for anti-CYB5R1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
34,095 Da
NCBI Official Full Name
Homo sapiens cytochrome b5 reductase 1, mRNA
UniProt Protein Name
NADH-cytochrome b5 reductase 1
Protein Family
UniProt Gene Name
CYB5R1
UniProt Synonym Gene Names
NQO3A2; b5R.1
UniProt Entry Name
NB5R1_HUMAN
Uniprot Description
CYB5R1: NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
Protein type: Oxidoreductase; EC 1.6.2.2; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Membrane protein, integral
Chromosomal Location of Human Ortholog: 1q32.1
Cellular Component: membrane; mitochondrion; integral to membrane
Molecular Function: cytochrome-b5 reductase activity
Biological Process: sterol biosynthetic process
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Product Notes
The CYB5R1 cyb5r1 (Catalog #AAA4758174) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CYB5R1 Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CYB5R1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:200-1:1000. Researchers should empirically determine the suitability of the CYB5R1 cyb5r1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CYB5R1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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