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Western Blot (WB) (Gel: 8%SDS-PAGE, Lysate: 60 ug, Lane: Mouse liver tissue, Primary antibody: 162448(UCP2 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes)

Rabbit anti-Human, Mouse UCP2 Polyclonal Antibody | anti-UCP2 antibody

UCP2 Rabbit pAb

Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
UCP2; Polyclonal Antibody; UCP2 Rabbit pAb; uncoupling protein 2 (mitochondrial; proton carrier); UCPH; BMIQ4; SLC25A8; anti-UCP2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Affinity purified
Form/Format
-20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Applicable Applications for anti-UCP2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:200-1:1000
IHC:10-1:50
Immunogen
Synthetic peptide of human UCP2
Conjugation
Unconjugated
Modification
Unmodified
Cell Position
Membrane, Mitochondrion, Mitochondrion inner membrane
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Gel: 8%SDS-PAGE, Lysate: 60 ug, Lane: Mouse liver tissue, Primary antibody: 162448(UCP2 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes)

Western Blot (WB) (Gel: 8%SDS-PAGE, Lysate: 60 ug, Lane: Mouse liver tissue, Primary antibody: 162448(UCP2 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 162448(UCP2 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200))

Immunohistochemistry (IHC) (The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 162448(UCP2 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200))
Product Categories/Family for anti-UCP2 antibody

NCBI and Uniprot Product Information

UniProt Accession #
Molecular Weight
33kDa
UniProt Protein Name
Mitochondrial uncoupling protein 2
UniProt Gene Name
UCP2
UniProt Synonym Gene Names
SLC25A8; UCP 2
UniProt Entry Name
UCP2_HUMAN

Uniprot Description

UCP2: UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. Belongs to the mitochondrial carrier family.

Protein type: Membrane protein, multi-pass; Mitochondrial; Membrane protein, integral; Transporter

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: mitochondrial inner membrane; integral to membrane

Biological Process: cellular metabolic process; proton transport; response to superoxide; regulation of mitochondrial membrane potential; cellular response to insulin stimulus; transport; cellular response to amino acid starvation; response to hypoxia; female pregnancy; negative regulation of apoptosis; aging; mitochondrial transport

Disease: Body Mass Index Quantitative Trait Locus 4

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Product Notes

The UCP2 ucp2 (Catalog #AAA4754926) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UCP2 Rabbit pAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's UCP2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:200-1:1000 IHC:10-1:50. Researchers should empirically determine the suitability of the UCP2 ucp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UCP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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