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Western Blot (WB) (Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: NIH/3T3 cells, Primary antibody: 162022(IVL Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds)

Rabbit anti-Human, Mouse Involucrin Polyclonal Antibody | anti-IVL antibody

Involucrin Rabbit pAb

Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
Involucrin; Polyclonal Antibody; Involucrin Rabbit pAb; involucrin; anti-IVL antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Keratinocytes of epidermis and other stratified squamous epithelia. Gene expression databases
Purity/Purification
Affinity purified
Form/Format
pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Applicable Applications for anti-IVL antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:200-1:1000
ELISA: 1:1000-1:2000
IHC: 1:50-1:200
Immunogen
Synthetic peptide of human IVL
Conjugation
Unconjugated
Molecular Function
Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
Modification
Unmodified
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: NIH/3T3 cells, Primary antibody: 162022(IVL Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds)

Western Blot (WB) (Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: NIH/3T3 cells, Primary antibody: 162022(IVL Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using 162022(IVL Antibody) at dilution 1/50. (Original magnification: ×200))

Immunohistochemistry (IHC) (The image is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using 162022(IVL Antibody) at dilution 1/50. (Original magnification: ×200))

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 162022(IVL Antibody) at dilution 1/50.(Original magnification: ×200))

Immunohistochemistry (IHC) (The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 162022(IVL Antibody) at dilution 1/50.(Original magnification: ×200))
Related Product Information for anti-IVL antibody
Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin.
Product Categories/Family for anti-IVL antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
68,479 Da
NCBI Official Full Name
involucrin
NCBI Official Synonym Full Names
involucrin
NCBI Official Symbol
IVL
NCBI Protein Information
involucrin
UniProt Protein Name
Involucrin
Protein Family
UniProt Gene Name
IVL
UniProt Entry Name
INVO_HUMAN

NCBI Description

Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin. [provided by RefSeq, Jul 2008]

Uniprot Description

IVL: Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia. Belongs to the involucrin family.

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: cytoplasm; cornified envelope

Molecular Function: protein binding, bridging; structural molecule activity

Biological Process: keratinocyte differentiation; isopeptide cross-linking via N6-(L-isoglutamyl)-L-lysine; keratinization; peptide cross-linking; response to UV-B

Research Articles on IVL

Similar Products

Product Notes

The IVL ivl (Catalog #AAA4754857) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Involucrin Rabbit pAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Involucrin can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:200-1:1000 ELISA: 1:1000-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the IVL ivl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Involucrin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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