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Western Blot (WB) (Western blot detection of RPL10A in K562,C6,3T3,Hela cell lysates using RPL10A Rabbit mAb(1:1000 diluted). Predicted band size:25kDa. Observed band size:25kDa.)

Rabbit RPL10A Monoclonal Antibody | anti-RPL10A antibody

RPL10A Rabbit mAb

Gene Names
RPL10A; L10A; NEDD6; Csa-19
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
Affinity purified
Synonyms
RPL10A; Monoclonal Antibody; RPL10A Rabbit mAb; 60S ribosomal protein L10a; L10A; CSA19; NEDD6; Csa-19; anti-RPL10A antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
R08-9H8
Purity/Purification
Affinity purified
Form/Format
Supplied in 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applicable Applications for anti-RPL10A antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000-1:5000
IHC: 1:20-1:100
ICC/IF: 1:20-1:50
FC/FACS: 1:20
Immunogen
Recombinant protein of human RPL10A
Conjugation
Unconjugated
Modification
Unmodification
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western blot detection of RPL10A in K562,C6,3T3,Hela cell lysates using RPL10A Rabbit mAb(1:1000 diluted). Predicted band size:25kDa. Observed band size:25kDa.)

Western Blot (WB) (Western blot detection of RPL10A in K562,C6,3T3,Hela cell lysates using RPL10A Rabbit mAb(1:1000 diluted). Predicted band size:25kDa. Observed band size:25kDa.)
Related Product Information for anti-RPL10A antibody
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L1P family of ribosomal proteins. It is located in the cytoplasm. The expression of this gene is downregulated in the thymus by cyclosporin-A (CsA), an immunosuppressive drug. Studies in mice have shown that the expression of the ribosomal protein L10a gene is downregulated in neural precursor cells during development. This gene previously was referred to as NEDD6 (neural precursor cell expressed, developmentally downregulated 6), but it has been renamed RPL10A (ribosomal protein 10a). As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
Component of the large ribosomal subunit.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24,831 Da
NCBI Official Full Name
60S ribosomal protein L10a
NCBI Official Synonym Full Names
ribosomal protein L10a
NCBI Official Symbol
RPL10A
NCBI Official Synonym Symbols
L10A; NEDD6; Csa-19
NCBI Protein Information
60S ribosomal protein L10a; NEDD-6; neural precursor cell expressed developmentally down-regulated protein 6
UniProt Protein Name
60S ribosomal protein L10a
Protein Family
UniProt Gene Name
RPL10A
UniProt Synonym Gene Names
NEDD6; NEDD-6
UniProt Entry Name
RL10A_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L1P family of ribosomal proteins. It is located in the cytoplasm. The expression of this gene is downregulated in the thymus by cyclosporin-A (CsA), an immunosuppressive drug. Studies in mice have shown that the expression of the ribosomal protein L10a gene is downregulated in neural precursor cells during development. This gene previously was referred to as NEDD6 (neural precursor cell expressed, developmentally downregulated 6), but it has been renamed RPL10A (ribosomal protein 10a). As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

RPL10A: a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L1P family of ribosomal proteins. It is located in the cytoplasm. The expression of this gene is downregulated in the thymus by cyclosporin-A (CsA), an immunosuppressive drug. Studies in mice have shown that the expression of the ribosomal protein L10a gene is downregulated in neural precursor cells during development. This gene previously was referred to as NEDD6 (neural precursor cell expressed, developmentally downregulated 6), but it has been renamed RPL10A (ribosomal protein 10a). As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Protein type: Translation; Ribosomal

Chromosomal Location of Human Ortholog: 6p21.31

Cellular Component: focal adhesion; mitochondrion; membrane; cytoplasm; nucleolus; cytosol; nucleus

Molecular Function: protein binding; structural constituent of ribosome

Biological Process: SRP-dependent cotranslational protein targeting to membrane; anatomical structure morphogenesis; cellular protein metabolic process; translational elongation; translation; viral reproduction; translational initiation; mRNA catabolic process, nonsense-mediated decay; viral transcription; gene expression; viral infectious cycle; translational termination

Research Articles on RPL10A

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Product Notes

The RPL10A rpl10a (Catalog #AAA4753006) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RPL10A Rabbit mAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RPL10A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS). WB: 1:1000-1:5000 IHC: 1:20-1:100 ICC/IF: 1:20-1:50 FC/FACS: 1:20. Researchers should empirically determine the suitability of the RPL10A rpl10a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPL10A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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