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Western Blot (WB) (Western blot detection of NDUFB9 in Rat Brain lysates using NDUFB9 Rabbit mAb(1:1000 diluted). Predicted band size:22kDa. Observed band size:22kDa.)

Rabbit NDUFB9 Monoclonal Antibody | anti-NDUFB9 antibody

NDUFB9 Rabbit mAb

Gene Names
NDUFB9; B22; LYRM3; UQOR22
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, Immunoprecipitation
Purity
Affinity purified
Synonyms
NDUFB9; Monoclonal Antibody; NDUFB9 Rabbit mAb; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9; B22; LYRM3; CI-B22; UQOR22; anti-NDUFB9 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
R05-9F9
Purity/Purification
Affinity purified
Form/Format
Supplied in 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applicable Applications for anti-NDUFB9 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes
WB: 1:1000
IHC: 1:50
ICC/IF: 1:50
FC/FACS: 1:50
IP: 1:20
Immunogen
Recombinant protein of human NDUFB9
Conjugation
Unconjugated
Modification
Unmodification
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western blot detection of NDUFB9 in Rat Brain lysates using NDUFB9 Rabbit mAb(1:1000 diluted). Predicted band size:22kDa. Observed band size:22kDa.)

Western Blot (WB) (Western blot detection of NDUFB9 in Rat Brain lysates using NDUFB9 Rabbit mAb(1:1000 diluted). Predicted band size:22kDa. Observed band size:22kDa.)
Related Product Information for anti-NDUFB9 antibody
The protein encoded by this gene is a subunit of the mitochondrial oxidative phosphorylation complex I (nicotinamide adenine dinucleotide: ubiquinone oxidoreductase). Complex I is localized to the inner mitochondrial membrane and functions to dehydrogenate nicotinamide adenine dinucleotide and to shuttle electrons to coenzyme Q. Complex I deficiency is the most common defect found in oxidative phosphorylation disorders and results in a range of conditions, including lethal neonatal disease, hypertrophic cardiomyopathy, liver disease, and adult-onset neurodegenerative disorders. Pseudogenes of this gene are found on chromosomes five, seven and eight. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
21,831 Da
NCBI Official Full Name
NADH dehydrogenase
NCBI Official Synonym Full Names
NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa
NCBI Official Symbol
NDUFB9
NCBI Official Synonym Symbols
B22; LYRM3; UQOR22
NCBI Protein Information
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9; CI-B22; complex I-B22; complex I B22 subunit; LYR motif-containing protein 3; NADH-ubiquinone oxidoreductase B22 subunit
UniProt Protein Name
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9
Protein Family
UniProt Gene Name
NDUFB9
UniProt Synonym Gene Names
LYRM3; UQOR22; CI-B22
UniProt Entry Name
NDUB9_HUMAN

NCBI Description

The protein encoded by this gene is a subunit of the mitochondrial oxidative phosphorylation complex I (nicotinamide adenine dinucleotide: ubiquinone oxidoreductase). Complex I is localized to the inner mitochondrial membrane and functions to dehydrogenate nicotinamide adenine dinucleotide and to shuttle electrons to coenzyme Q. Complex I deficiency is the most common defect found in oxidative phosphorylation disorders and results in a range of conditions, including lethal neonatal disease, hypertrophic cardiomyopathy, liver disease, and adult-onset neurodegenerative disorders. Pseudogenes of this gene are found on chromosomes five, seven and eight. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

Uniprot Description

NDUFB9: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I LYR family.

Protein type: EC 1.6.99.3; Mitochondrial; Energy Metabolism - oxidative phosphorylation; Oxidoreductase; EC 1.6.5.3

Chromosomal Location of Human Ortholog: 8q13.3

Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial respiratory chain complex I

Molecular Function: NADH dehydrogenase (ubiquinone) activity

Biological Process: cellular metabolic process; sensory perception of sound; mitochondrial electron transport, NADH to ubiquinone

Disease: Mitochondrial Complex I Deficiency

Research Articles on NDUFB9

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Product Notes

The NDUFB9 ndufb9 (Catalog #AAA4752900) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NDUFB9 Rabbit mAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NDUFB9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP). WB: 1:1000 IHC: 1:50 ICC/IF: 1:50 FC/FACS: 1:50 IP: 1:20. Researchers should empirically determine the suitability of the NDUFB9 ndufb9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NDUFB9, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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