Western Blot (WB)
(Western blot detection of ACOX1/AOX in Raw264.7,Mouse Liver cell lysates using ACOX1/AOX Rabbit mAb(1:500 diluted). Predicted band size:74kDa. Observed band size:74,48kDa.)
Rabbit ACOX1/AOX Monoclonal Antibody | anti-Acox1 antibody
ACOX1/AOX Rabbit mAb
Gene Names
Acox1; AOX; Acox; Paox; D130055E20Rik
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, Immunoprecipitation
Purity
Affinity purified
Synonyms
ACOX1/AOX; Monoclonal Antibody; ACOX1/AOX Rabbit mAb; AOX; Acox; Paox; D130055E20Rik; anti-Acox1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
Rabbit IgG
Specificity
Highest levels of isoform 1 are found in liver and kidney while highest levels of isoform 2 are found in white adipose tissue. Isoform 1 is expressed at higher levels than isoform 2 in liver and kidney while isoform 2 is expressed at higher levels in brain, heart, lung, muscle, white adipose tissue and testis.
Purity/Purification
Affinity purified
Form/Format
50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applicable Applications for anti-Acox1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes
WB: 1:1000
ICC/IF: 1:50
FC/FACS: 1:20
IP: 1:20
ICC/IF: 1:50
FC/FACS: 1:20
IP: 1:20
Immunogen
Recombinant protein of mouse ACOX1
Conjugation
Unconjugated
Modification
Unmodification
Preparation and Storage
Store at 4 degree C short term. Store at -20 degree C long term. Avoid freeze/thaw cycle.
Western Blot (WB)
(Western blot detection of ACOX1/AOX in Raw264.7,Mouse Liver cell lysates using ACOX1/AOX Rabbit mAb(1:500 diluted). Predicted band size:74kDa. Observed band size:74,48kDa.)
Western Blot (WB)
(Western blot detection of ACOX1/AOX in Raw264.7,Mouse Liver cell lysates using ACOX1/AOX Rabbit mAb(1:500 diluted). Predicted band size:74kDa. Observed band size:74,48kDa.)
Related Product Information for anti-Acox1 antibody
This gene encodes a member of the acyl-coenzyme A oxidase family. The encoded protein is localized to peroxisomes and is the first enzyme of the fatty acid beta-oxidation pathway, which catalyzes the desaturation of acyl-coenzyme A to 2-trans-enoyl-coenzyme A. Disruption of this gene results in microvesicular steatohepatitis, spontaneous peroxisome proliferation, and the eventual development of hepatocellular carcinomas. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]
Catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. Isoform 1 shows highest activity against medium-chain fatty acyl-CoAs and activity decreases with increasing chain length. Isoform 2 is active against a much broader range of substrates and shows activity towards very long-chain acyl-CoAs.
Catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. Isoform 1 shows highest activity against medium-chain fatty acyl-CoAs and activity decreases with increasing chain length. Isoform 2 is active against a much broader range of substrates and shows activity towards very long-chain acyl-CoAs.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
74,649 Da
NCBI Official Full Name
peroxisomal acyl-coenzyme A oxidase 1 isoform 2
NCBI Official Synonym Full Names
acyl-Coenzyme A oxidase 1, palmitoyl
NCBI Official Symbol
Acox1
NCBI Official Synonym Symbols
AOX; Acox; Paox; D130055E20Rik
NCBI Protein Information
peroxisomal acyl-coenzyme A oxidase 1; palmitoyl-CoA oxidase; peroxisomal fatty acyl-coenzyme A oxidase; peroxisomal straight-chain Acyl-CoA oxidase
UniProt Protein Name
Peroxisomal acyl-coenzyme A oxidase 1
Protein Family
UniProt Gene Name
Acox1
UniProt Synonym Gene Names
Acox; Paox; AOX
UniProt Entry Name
ACOX1_MOUSE
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Product Notes
The Acox1 acox1 (Catalog #AAA4752557) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ACOX1/AOX Rabbit mAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ACOX1/AOX can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP). WB: 1:1000 ICC/IF: 1:50 FC/FACS: 1:20 IP: 1:20. Researchers should empirically determine the suitability of the Acox1 acox1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACOX1/AOX, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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