Rabbit FOXC1 Polyclonal Antibody | anti-FOXC1 antibody

FOXC1 Rabbit pAb

Cat. Num. AAA4751967

• Gene Names: FOXC1; ARA; IGDA; IHG1; FKHL7; IRID1; RIEG3; FREAC3; FREAC-3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, Immunoprecipitation
• Purity: Affinity purified
• Synonyms: FOXC1; Polyclonal Antibody; FOXC1 Rabbit pAb; Forkhead box protein C1; ARA; IGDA; IHG1; ASGD3; FKHL7; IRID1; RIEG3; FREAC3; FREAC-3; anti-FOXC1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Expressed in keratinocytes of epidermis and hair follicle (PubMed:27907090). Expressed strongly in microvascular invasion (MVI) formation, basal-like breast cancer (BLBC) and hepatocellular tumors (PubMed:20406990, PubMed:22991501). Expressed in breast cancers (at protein level) (PubMed:26565916). Expressed in hematopoietic cells (PubMed:8499623).
• Purity/Purification: Affinity purified
• Form/Format: Supplied in 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

• Applicable Applications for anti-FOXC1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
• Application Notes: WB: 1:1000; IHC: 1:200; ICC/IF: 1:100; FC/FACS: 1:100; IP: 1:20
• Immunogen: Recombinant protein of human FOXC1
• Conjugation: Unconjugated
• Modification: Unmodification
• Subcellular Location: Nucleus
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot detection of FOXC1 in 3T3 cell lysates using FOXC1 Rabbit pAb(1:1000 diluted). Predicted band size:57kDa. Observed band size:75kDa.)

Related Product Information for anti-FOXC1 antibody

This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it has been shown to play a role in the regulation of embryonic and ocular development. Mutations in this gene cause various glaucoma phenotypes including primary congenital glaucoma, autosomal dominant iridogoniodysgenesis anomaly, and Axenfeld-Rieger anomaly. [provided by RefSeq, Jul 2008]
DNA-binding transcriptional factor that plays a role in a broad range of cellular and developmental processes such as eye, bones, cardiovascular, kidney and skin development (PubMed:11782474, PubMed:15299087, PubMed:15684392, PubMed:16492674, PubMed:27907090, PubMed:14506133, PubMed:14578375, PubMed:15277473, PubMed:16449236, PubMed:17210863, PubMed:19793056, PubMed:19279310, PubMed:25786029, PubMed:27804176). Acts either as a transcriptional activator or repressor (PubMed:11782474). Binds to the consensus binding site 5'-[G/C][A/T]AAA[T/C]AA[A/C]-3' in promoter of target genes (PubMed:7957066, PubMed:11782474, PubMed:12533514, PubMed:14506133, PubMed:19793056, PubMed:27804176). Upon DNA-binding, promotes DNA bending (PubMed:7957066, PubMed:14506133). Acts as a transcriptional coactivator (PubMed:26565916). Stimulates Indian hedgehog (Ihh)-induced target gene expression mediated by the transcription factor GLI2, and hence regulates endochondral ossification. Acts also as a transcriptional coregulator by increasing DNA-binding capacity of GLI2 in breast cancer cells (PubMed:26565916). Regulates FOXO1 through binding to a conserved element, 5'-GTAAACAAA-3' in its promoter region, implicating FOXC1 as an important regulator of cell viability and resistance to oxidative stress in the eye (PubMed:17993506). Cooperates with transcription factor FOXC2 in regulating expression of genes that maintain podocyte integrity. Promotes cell growth inhibition by stopping the cell cycle in the G1 phase through TGFB1-mediated signals (PubMed:12408963). Involved in epithelial-mesenchymal transition (EMT) induction by increasing cell proliferation, migration and invasion (PubMed:20406990, PubMed:22991501). Involved in chemokine CXCL12-induced endothelial cell migration through the control of CXCR4 expression. Plays a role in the gene regulatory network essential for epidermal keratinocyte terminal differentiation (PubMed:27907090). Essential developmental transcriptional factor required for mesoderm-derived tissues, such as the somites, skin, bone and cartilage. Positively regulates CXCL12 and stem cell factor expression in bone marrow mesenchymal progenitor cells, and hence plays a role in the development and maintenance of mesenchymal niches for haematopoietic stem and progenitor cells (HSPC). Plays a role in corneal transparency by preventing both blood vessel and lymphatic vessel growth during embryonic development in a VEGF-dependent manner. Involved in chemokine CXCL12-induced endothelial cell migration through the control of CXCR4 expression. May function as a tumor suppressor (PubMed:12408963).

NCBI and Uniprot Product Information

• NCBI GI #: 119395716
• NCBI GeneID: 2296
• NCBI Accession #: NP_001444.2
• NCBI GenBank Nucleotide #: NM_001453.2
• UniProt Accession #: Q12948; Q86UP7; Q9BYM1; Q9NUE5; Q9UDD0; Q9UP06
• Molecular Weight: 56,789 Da
• NCBI Official Full Name: forkhead box protein C1
• NCBI Official Synonym Full Names: forkhead box C1
• NCBI Official Symbol: FOXC1
• NCBI Official Synonym Symbols: ARA; IGDA; IHG1; FKHL7; IRID1; RIEG3; FREAC3; FREAC-3
• NCBI Protein Information: forkhead box protein C1; myeloid factor-delta; forkhead-related activator 3; forkhead-related protein FKHL7; forkhead, drosophila, homolog-like 7; forkhead-related transcription factor 3; forkhead/winged helix-like transcription factor 7
• UniProt Protein Name: Forkhead box protein C1
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXC1
• UniProt Synonym Gene Names: FKHL7; FREAC3; FREAC-3
• UniProt Entry Name: FOXC1_HUMAN

NCBI Description

This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it has been shown to play a role in the regulation of embryonic and ocular development. Mutations in this gene cause various glaucoma phenotypes including primary congenital glaucoma, autosomal dominant iridogoniodysgenesis anomaly, and Axenfeld-Rieger anomaly. [provided by RefSeq, Jul 2008]

Uniprot Description

FOXC1: Binding of FREAC-3 and FREAC-4 to their cognate sites results in bending of the DNA at an angle of 80-90 degrees. Monomer. Expressed in all tissues and cell lines examined.

Protein type: Transcription factor; Cell development/differentiation; DNA-binding; Cell cycle regulation

Chromosomal Location of Human Ortholog: 6p25

Cellular Component: nucleoplasm; cytoplasm; nuclear heterochromatin; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; DNA binding; sequence-specific DNA binding; transcription factor binding; DNA bending activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; collagen fibril organization; neural crest cell development; glycosaminoglycan metabolic process; paraxial mesoderm formation; positive regulation of transcription, DNA-dependent; heart development; cardiac muscle cell proliferation; ovarian follicle development; regulation of transcription, DNA-dependent; ureteric bud development; germ cell migration; ventricular cardiac muscle morphogenesis; negative regulation of mitotic cell cycle; lacrimal gland development; regulation of blood vessel size; skeletal development; mesenchymal cell development; ossification; somitogenesis; Notch signaling pathway; cell migration; camera-type eye development; in utero embryonic development; regulation of organ growth; odontogenesis of dentine-containing teeth; cell proliferation; eye development; artery morphogenesis; embryonic heart tube development; blood vessel remodeling; positive regulation of transcription from RNA polymerase II promoter; brain development; vascular endothelial growth factor receptor signaling pathway; lymph vessel development

Disease: Axenfeld-rieger Syndrome, Type 3; Iridogoniodysgenesis, Type 1