Western Blot (WB)
(Western blot detection of c-JUN in CHO-K1 transfected by PEGFP-C1-c-JUN (A)and CHO-K1 cell lysate (B)cell lysate using c-JUN mouse mAb (1:1000 diluted).Predicted band size:43/48KDa.Observed band size:43/48KDa.)
c-JUN Monoclonal Antibody | anti-c-JUN antibody
Anti-c-JUN Mouse mAb
Gene Names
JUN; AP1; AP-1; c-Jun
Applications
Western Blot
Purity
Affinity Purified
Synonyms
c-JUN; Monoclonal Antibody; Anti-c-JUN Mouse mAb; Activator Protein 1; AP 1; AP1; cJun; Enhancer Binding Protein AP1; Jun Activation Domain Binding Protein; JUN; Jun oncogene; JUN protein; Jun proto oncogene; JUN_HUMAN; JUNC; Oncogene JUN; p39; Proto oncogene c jun; Proto oncogene cJun; Proto-oncogene c-jun; Transcription Factor AP 1; Transcription factor AP-1; Transcription Factor AP1; V jun avian sarcoma virus 17 oncogene homolog; V jun sarcoma virus 17 oncogene homolog (avian); V jun sarcoma virus 17 oncogene homolog; V-jun avian sarcoma virus 17 oncogene homolog; vJun Avian Sarcoma Virus 17 Oncogene Homolog; anti-c-JUN antibody
Host
Mouse
Source: Human
Source: Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3B3-A5-D7-F7
Purity/Purification
Affinity Purified
Form/Format
Purified mouse monoclonal in PBS (pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Concentration
3.0mg/ml (varies by lot)
Sequence Length
331
Applicable Applications for anti-c-JUN antibody
Western Blot (WB)
Application Notes
WB: 1:1000
Antibody Type
Primary antibody
Conjugation
Unconjugated
Modification
Unmodification
Immunogen
Purified recombinant human c-JUN protein fragments expressed in E Coli.
Sensitivity
Transfected
Sequence Similarities
Belongs to the bZIP family. Jun subfamily.Contains 1 bZIP (basic-leucine zipper) domain.
Subunit Structure
Heterodimer with either FOS or BATF3 or ATF7.The ATF7/JUN heterodimer is essential for ATF7 transactivation activity.Interacts with DSIPI; the interaction inhibits the binding of active AP1 to its target DNA by similarity.Interacts with HIVEP3 and MYBBP1A by similarity.Interacts with SP1, SPIB and TCF20.Interacts with COPS5; the interaction leads indirectly to its phosphorylation.Component of the SMAD3/SMAD4/JUN/FOS/complex which forms at the AP1 promoter site.The SMAD3/SMAD4 heterodimer acts syngernistically with the JUN/FOS heterodimer to activate transcription in response to TGF-beta.Interacts (via its basic DNA binding and leucine zipper domains) with SMAD3 (via an N-terminal domain); the interaction is required for TGF-beta-mediated transactivation of the SMAD3/SMAD4/JUN/FOS/complex.Interacts with methylated RNF187.Binds to HIPK3.
Subcellular Location
Nucleus.
Preparation and Storage
Store at 4 degree C short term. Store at -20 degree C long term. Avoid freeze/thaw cycle.
Western Blot (WB)
(Western blot detection of c-JUN in CHO-K1 transfected by PEGFP-C1-c-JUN (A)and CHO-K1 cell lysate (B)cell lysate using c-JUN mouse mAb (1:1000 diluted).Predicted band size:43/48KDa.Observed band size:43/48KDa.)
Western Blot (WB)
(Western blot detection of c-JUN in CHO-K1 transfected by PEGFP-C1-c-JUN (A)and CHO-K1 cell lysate (B)cell lysate using c-JUN mouse mAb (1:1000 diluted).Predicted band size:43/48KDa.Observed band size:43/48KDa.)
Related Product Information for anti-c-JUN antibody
Entrez Summary: This gene is the putative transforming gene of avian sarcoma virus 17.It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression.This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.[provided by RefSeq, Jul 2008]
UniPort Summary Function: Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.
UniPort Summary Function: Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43/48 kDa
NCBI Official Full Name
transcription factor AP-1
NCBI Official Synonym Full Names
Jun proto-oncogene, AP-1 transcription factor subunit
NCBI Official Symbol
JUN
NCBI Official Synonym Symbols
AP1; AP-1; c-Jun
NCBI Protein Information
transcription factor AP-1
UniProt Protein Name
Transcription factor AP-1
Protein Family
UniProt Gene Name
JUN
UniProt Synonym Gene Names
AP1
NCBI Description
This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008]
Uniprot Description
Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).
Research Articles on c-JUN
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Product Notes
The c-JUN jun (Catalog #AAA475094) is an Antibody produced from Mouse Source: Human and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's c-JUN can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000. Researchers should empirically determine the suitability of the c-JUN jun for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "c-JUN, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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