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Western Blot (WB) (Western blot analysis of 14-3-3 gamma expression in HeLa cell lysate.)

Rabbit 14-3-3 gamma Polyclonal Antibody | anti-YWHAG antibody

Anti-14-3-3 gamma Rabbit pAb

Gene Names
YWHAG; EIEE56; PPP1R170; 14-3-3GAMMA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry, Functional Assay
Purity
Affinity Chromatography
Synonyms
14-3-3 gamma; Polyclonal Antibody; Anti-14-3-3 gamma Rabbit pAb; 1433G; 143G; KCIP-1; Protein kinase C inhibitor protein-1; gamma polypeptide; anti-YWHAG antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
14-3-3 gamma Antibody detects endogenous levels of 14-3-3 gamma
Purity/Purification
Affinity Chromatography
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
247
Applicable Applications for anti-YWHAG antibody
Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000-1:2000
FC/FACS: 1:50
Immunogen
A synthesized peptide derived from human 14-3-3 gamma
Subcellular Location
Nucleus
Preparation and Storage
Store at 4 degree C for short term.
Store at-20 degree C for long term.
Avoid freeze/thaw cycle.

Western Blot (WB)

(Western blot analysis of 14-3-3 gamma expression in HeLa cell lysate.)

Western Blot (WB) (Western blot analysis of 14-3-3 gamma expression in HeLa cell lysate.)
Related Product Information for anti-YWHAG antibody
Induce target protein conformational changes that modify target protein function. Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in development and in acute response to extracellular signals and drugs, suggesting that 14-3-3 isoforms may perform different functions despite their sequence similarities.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
28kDa
NCBI Official Full Name
14-3-3 protein gamma
NCBI Official Synonym Full Names
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma
NCBI Official Symbol
YWHAG
NCBI Official Synonym Symbols
EIEE56; PPP1R170; 14-3-3GAMMA
NCBI Protein Information
14-3-3 protein gamma
UniProt Protein Name
14-3-3 protein gamma
Protein Family
UniProt Gene Name
YWHAG
UniProt Synonym Gene Names
KCIP-1

NCBI Description

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq, Jul 2008]

Uniprot Description

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

Research Articles on YWHAG

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Product Notes

The YWHAG ywhag (Catalog #AAA4750576) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-14-3-3 gamma Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's 14-3-3 gamma can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS). WB: 1:1000-1:2000 FC/FACS: 1:50. Researchers should empirically determine the suitability of the YWHAG ywhag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "14-3-3 gamma, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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