PKC (alpha,beta,gamma) Phospho-Regulation Antibody Sampler Kit | PKC antibody sampler kit
PKC (alpha,beta,gamma) Phospho-Regulation
Reactivity
Human, Rat, Mouse
Applications
Western Blot, ELISA, Immunoprecipitation, Immunocytochemistry
Synonyms
PKC (alpha; beta; gamma) Phospho-Regulation; PKC antibody sampler kit
Reactivity
Human, Rat, Mouse
Form/Format
Mouse monoclonal and rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide.
Applicable Applications for PKC antibody sampler kit
Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), Immunocytochemistry (ICC)
Preparation and Storage
Store all at -20 degree C.Stable for 1 year.
Related Product Information for PKC antibody sampler kit
Kit Summary: The PKC (alpha, beta, gamma) phospho-regulation kit can be used to examine phosphorylation of PKCalpha (Ser-657/Tyr-658) relative tothe total expression of PKCalpha. The phospho-specific antibody also detects phosphorylation of the the conserved dual sitein PKCbeta and PKCgamma. The kit includes mouse monoclonal antibodies to detect total PKCalpha and PKC (alpha, beta, gamma), as well assecondary reagents for rabbit polyclonal and mouse monoclonal antibody detection.
Background: The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processessuch as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. PKC consists of asingle polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half interacts with PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester, while the C-terminal half contains the catalyticdomain. The conventional PKC subfamily (alpha, beta1, betaII, and gamma) is regulated by both Ca2+ and diacylglycerol. The PKCpathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters and growth factors. The phosphorylation of multiple sites in conventional PKCsregulates their activity. In mast cells, FceRI stimulation leads to phosphorylation of tyrosine 658 and 662 of PKCalpha and PKCbetaI respectively. This phosphorylation requires autophosphorylation of serine 657 and 661 in these respective kinases.
Background: The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processessuch as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. PKC consists of asingle polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half interacts with PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester, while the C-terminal half contains the catalyticdomain. The conventional PKC subfamily (alpha, beta1, betaII, and gamma) is regulated by both Ca2+ and diacylglycerol. The PKCpathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters and growth factors. The phosphorylation of multiple sites in conventional PKCsregulates their activity. In mast cells, FceRI stimulation leads to phosphorylation of tyrosine 658 and 662 of PKCalpha and PKCbetaI respectively. This phosphorylation requires autophosphorylation of serine 657 and 661 in these respective kinases.
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Product Notes
The Human, Rat, Mouse PKC (Catalog #AAA474493) is an Antibody Sampler Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA474493 Antibody Sampler Kit recognizes Human, Rat, Mouse PKC. AAA Biotech's PKC (alpha,beta,gamma) Phospho-Regulation can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), Immunocytochemistry (ICC). Researchers should empirically determine the suitability of the PKC for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PKC (alpha,beta,gamma) Phospho-Regulation, Antibody Sampler Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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