Mouse Hsp60 Monoclonal Antibody | anti-Hsp60 antibody

Hsp60 (N-terminal region) Antibody

Cat. Num. AAA474377

• Gene Names: HSPD1; HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, ELISA, Immunocytochemistry
• Purity: Protein A chromatography
• Synonyms: Hsp60; Monoclonal Antibody; Hsp60 (N-terminal region) Antibody; Hsp60 (N-terminal region); anti-Hsp60 antibody

• Host: Mouse
• Reactivity: Human, Rat, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: M438
• Specificity: This antibody detects a 60kDa* protein corresponding to the apparent molecular mass of Hsp60 on SDS-PAGE immunoblots of human Jurkat and rat A7r5 cells. In immunocytochemistry, anti-Hsp60 specifically stains mitochondria in paraformaldehyde fixed and NP-40 permeabilized cells.
• Purity/Purification: Protein A chromatography
• Form/Format: Mouse monoclonal purified with protein A chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-Hsp60 antibody: Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC)
• Application Notes: ELISA: 1:2000; ICC: 1:100; WB: 1:1000
• Immunogen: Clone M438 was generated from a recombinant protein corresponding to amino acid residues in the N-terminal region of human Hsp60. This sequence has high homology to similar regions in rat and mouse Hsp60
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Immunocytochemistry (ICC)

(Immunocytochemical labeling of Hsp60 in mitochondria in paraformaldehyde-fixed and NP40-permeabilized A7r5 cells. The cells were labeled with mouse monoclonal Hsp60 (HM4381). The antibody was detected using goat anti-mouse DyLight 488.)

Western Blot (WB)

(Western blot image of cell structure markers in NCI-H1915 lung carcinoma cells. The blot was probed with anti-Vimentin intermediate filament protein VM4341 (lane 1), anti-Nucleoporin p62nM4361 (lane 2), anti-Hsp60 mitochondrial protein HM4381 (lane 3), and anti-Calnexin endoplasmic reticulum protein CM4371 (lane 4).)

Related Product Information for anti-Hsp60 antibody

Heat shock proteins (Hsp) are a family of highly conserved proteins that include both constitutively expressed (Hsp60, Hsp70, and Hsp90) and stress-induced (Hsp27 and Hsp72) proteins. Hsp60 is a mitochondrial protein that promotes protein folding and facilitates proteolytic degradation of misfolded or denatured proteins in the mitochondria. Hsp10 interacts with Hsp60 to regulate its substrate binding and ATPase activity. In HeLa and Jurkat mitochondria, Hsp60 associates with caspase-3 to form a complex that dissociates and releases from the mitochondria during apoptosis. Hsp60 accelerates the maturation of procaspase-3 through its ATP-dependent "foldase" activity. In addition to its protein folding activity, Hsp60 can bind the toll-like receptor-4 complex leading to production of TNFalpha and stimulation of a pro-inflammatory response in macrophages. Thus, the protein folding function of Hsp60 is involved in protein folding in both normal and apoptotic cells, while release of Hsp60 during necrosis is thought to stimulate a pro-inflammatory response.

References

Grundtman, C. et al. (2011) Arterioscler Thromb Vasc Biol. 31(5):960.
Cappello, F. et al. (2008) Cancer Biol Ther. 7(6):801.
Venner, T.J. et al. (1990) DNA Cell Biol. 9(8):545.

NCBI and Uniprot Product Information

• NCBI GI #: 31542947
• NCBI GeneID: 3329
• NCBI Accession #: NP_002147.2
• NCBI GenBank Nucleotide #: NM_002156.4
• UniProt Accession #: P10809; Q38L19; Q9UCR6; B2R5M6
• Molecular Weight: 61,055 Da
• NCBI Official Full Name: 60 kDa heat shock protein, mitochondrial
• NCBI Official Synonym Full Names: heat shock 60kDa protein 1 (chaperonin)
• NCBI Official Symbol: HSPD1
• NCBI Official Synonym Symbols: HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
• NCBI Protein Information: 60 kDa heat shock protein, mitochondrial; chaperonin 60; 60 kDa chaperonin; heat shock protein 65; P60 lymphocyte protein; mitochondrial matrix protein P1; short heat shock protein 60 Hsp60s1
• UniProt Protein Name: 60 kDa heat shock protein, mitochondrial
• Protein Family: Hydrogenosomal chaperonin
• UniProt Gene Name: HSPD1
• UniProt Synonym Gene Names: HSP60; CPN60; HSP-60; Hsp60
• UniProt Entry Name: CH60_HUMAN

NCBI Description

This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010]

Uniprot Description

HSP60: Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. Interacts with HRAS. Interacts with HBV protein X and HTLV-1 protein p40tax. Belongs to the chaperonin (HSP60) family.

Protein type: Chaperone; Mitochondrial

Chromosomal Location of Human Ortholog: 2q33.1

Cellular Component: extracellular space; protein complex; cell surface; mitochondrion; early endosome; coated pit; cytosol; secretory granule; membrane; mitochondrial matrix; cytoplasm; mitochondrial inner membrane; plasma membrane; lipopolysaccharide receptor complex; cyclin-dependent protein kinase activating kinase holoenzyme complex

Molecular Function: protein binding; lipopolysaccharide binding; p53 binding; ubiquitin protein ligase binding; chaperone binding; unfolded protein binding; double-stranded RNA binding; ATPase activity; DNA replication origin binding; ATP binding; single-stranded DNA binding

Biological Process: caspase activation; B cell proliferation; viral reproduction; B cell activation; protein stabilization; T cell activation; positive regulation of interleukin-12 production; positive regulation of apoptosis; protein maturation; isotype switching to IgG isotypes; positive regulation of interleukin-6 production; B cell cytokine production; positive regulation of interleukin-10 production; response to unfolded protein; MyD88-dependent toll-like receptor signaling pathway; positive regulation of interferon-gamma production; 'de novo' protein folding; protein refolding; positive regulation of T cell mediated immune response to tumor cell; positive regulation of T cell activation; chaperone-mediated protein complex assembly; positive regulation of interferon-alpha production; negative regulation of apoptosis; positive regulation of macrophage activation

Disease: Spastic Paraplegia 13, Autosomal Dominant; Leukodystrophy, Hypomyelinating, 4