Monkey Soluble Fms-Like Tyrosine Kinase Receptor 1 ELISA Kit | SFLT-1 elisa kit

Monkey Soluble Fms-Like Tyrosine Kinase Receptor 1 ELISA Kit

Cat. Num. AAA47125

• Gene Names: Flt1; Flt-1; sFlt1; VEGFR1; VEGFR-1; AI323757
• Reactivity: Monkey
• Synonyms: Soluble Fms-Like Tyrosine Kinase Receptor 1; Monkey Soluble Fms-Like Tyrosine Kinase Receptor 1 ELISA Kit; SFLT-1 elisa kit

• Reactivity: Monkey
• Specificity: No significant cross-reactivity or interference between Monkey SFLT-1 and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 31.2pg/ml-1000pg/ml.
• Sensitivity: 5.0pg/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated SFLT-1 concentrations in Monkey serum, plasma and other body fluids. Using Purified Monkey SFLT-1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SFLT-1 and SFLT-1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SFLT-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for SFLT-1 elisa kit

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated SFLT-1 concentrations in Monkey serum, plasma and other body fluids. Using Purified Monkey SFLT-1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SFLT-1 and SFLT-1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SFLT-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 2809071
• NCBI GeneID: 14254
• UniProt Accession #: P35969; O55094; Q61517
• Molecular Weight: 149,876 Da
• NCBI Official Full Name: sFlt-1
• NCBI Official Synonym Full Names: FMS-like tyrosine kinase 1
• NCBI Official Symbol: Flt1
• NCBI Official Synonym Symbols: Flt-1; sFlt1; VEGFR1; VEGFR-1; AI323757
• NCBI Protein Information: vascular endothelial growth factor receptor 1; embryonic receptor kinase 2; tyrosine-protein kinase receptor FLT; vascular permeability factor receptor; vascular endothelial growth factor receptor-1
• UniProt Protein Name: Vascular endothelial growth factor receptor 1
• UniProt Gene Name: Flt1
• UniProt Synonym Gene Names: Emrk2; Flt; Vegfr1; VEGFR-1; FLT-1
• UniProt Entry Name: VGFR1_MOUSE

Uniprot Description

VEGFR1: a receptor tyrosine kinase of the VEGFR family. Receptor for VEGF, VEGFB and PGF. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Two splice variant isoforms have been described. Isoform SFlt1 may have an inhibitory role in angiogenesis.

Protein type: Protein kinase, tyrosine (receptor); EC 2.7.10.1; Membrane protein, integral; Kinase, protein; Protein kinase, TK; TK group; VEGFR family

Cellular Component: focal adhesion; membrane; integral to plasma membrane; integral to membrane; plasma membrane; receptor complex; endosome

Molecular Function: transferase activity; identical protein binding; vascular endothelial growth factor receptor activity; protein-tyrosine kinase activity; growth factor binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; transmembrane receptor protein tyrosine kinase activity; kinase activity; ATP binding; protein kinase activity

Biological Process: cell migration; peptidyl-tyrosine phosphorylation; multicellular organismal development; protein amino acid autophosphorylation; positive regulation of phosphoinositide 3-kinase activity; chemotaxis; protein amino acid phosphorylation; positive regulation of vascular endothelial growth factor receptor signaling pathway; patterning of blood vessels; positive regulation of phosphoinositide 3-kinase cascade; monocyte chemotaxis; positive regulation of MAP kinase activity; positive regulation of angiogenesis; positive regulation of MAPKKK cascade; response to hypoxia; blood vessel morphogenesis; embryonic morphogenesis; angiogenesis; sprouting angiogenesis; cell differentiation; vascular endothelial growth factor receptor signaling pathway; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cell migration