Testing Data
Rabbit Shaker Polyclonal Antibody
Rabbit anti Shaker
Reactivity
Drosophila, Insect
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
The rabbit IgG is purified by Epitope Affinity Purification.
Synonyms
Shaker; Polyclonal Antibody; Rabbit anti Shaker; Rabbit anti ST6Gal Polyclonal antibody; Sialyltransferase; ST6Gal; Beta-galactosidase alpha-2; 6 sialyltransferase;; anti-Shaker antibody
Host
Rabbit
Reactivity
Drosophila, Insect
Clonality
Polyclonal
Specificity
This antibody recognizes ~47 kDa of mouse FAH protein. The other species are not tested.
Purity/Purification
The rabbit IgG is purified by Epitope Affinity Purification.
Form/Format
This affinity purified antibody is supplied in sterile phosphatebuffered saline (pH7.2) containing antibody stabilizer
Concentration
100 ug/200 ul (varies by lot)
Applicable Applications for anti-Shaker antibody
ELISA, Western Blot, Immunohistochemistry
Application Notes
Suggested Dilution:
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Antigen Preparation
A synthetic peptide derived from the internal domain of Sialyltransferase protein from drosophila origin
Positive Control
Recombinant protein
Cellular Localization
Membrane
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C to 8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Testing Data
Testing Data
Related Product Information for anti-Shaker antibody
Sialyltransferases are enzyme that transfer sialic acid to nascent oligosaccharide. Each silaytransferase is specific for a particular sugar substrate. Sialytransferases add sialic acid to the terminal portins of the sialylated glycolipids or to the N-or O-linked sugar chases of glycoproteins. The animal sialyltransferases are Golgi type II transmembrane glycosyltransferases. While the enzyme ST6Gal I uses the Gal beta 1-4GlcNAc-R as the acceptor substrate which present in insects.
References
• Anne Harduin-Lepers, et al. The animal sialyltransferases and sialyltransferase-related genes: a phylogenetic approach. Glycobiology 2005 15(8):805-817
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Product Notes
The Shaker (Catalog #AAA462129) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Shaker reacts with Drosophila, Insect and may cross-react with other species as described in the data sheet. AAA Biotech's Shaker can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot, Immunohistochemistry. Suggested Dilution: Western Blot: 0.1-1 ug/ml ELISA: 0.01-0.1 ug/ml Immunoprecipitation: 2-5 ug/ml. Researchers should empirically determine the suitability of the Shaker for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Shaker, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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