Rabbit Osteopontin Polyclonal Antibody | anti-SPP1 antibody

Rabbit anti Osteopontin (SPP1) Polyclonal Antibody

Cat. Num. AAA460337

• Gene Names: SPP1; OPN; BNSP; BSPI; ETA-1
• Reactivity: Rat, Mouse, Human
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Epitope Affinity Purification
• Synonyms: Osteopontin; Polyclonal Antibody; Rabbit anti Osteopontin (SPP1) Polyclonal Antibody; SPP1; Secreted phosphoprotein; anti-SPP1 antibody

• Host: Rabbit
• Reactivity: Rat, Mouse, Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody recognizes ~34 kDa of Osteopontin protein. It reacts with human, mouse and rat. The other species are not tested.
• Purity/Purification: Purified by Epitope Affinity Purification
• Form/Format: Supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Sequence Length: 300

• Applicable Applications for anti-SPP1 antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: ELISA: 0.01-0.1 ug/ml; Flow Cytometry: 5-10 ug/ml; Immunohistochemistry: 2-10 ug/ml; Immunoprecipitation: 2-5 ug/ml; Western Blot: 0.1-1 ug/ml
• Preparation: A synthetic peptide corresponding to the internal portion of Osteopontin protein sequence. This sequence is identical to human, rat and mouse origins.
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20 degree C. The antibodies can be stored at 2 degree C - 8 degree C for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Related Product Information for anti-SPP1 antibody

Osteopontin (OPN or Early T-Lymphocyte Activation-1: Eta-1), also referred to as transformation-associated secreted phosphoprotein, bone sialoprotein I, and minopotin, is a highly phosphorylated and glycosylated phosphoprtein that is expressed in many tissues. It is acidic, calcium-binding, cell surface-binding sequences (RGD)-containing protein was originally isolated from bone matrix. Osteopontin has been found in kidney, placenta, blood vessels. It is over-expressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.

References

Oldberg, A., Franzen, A. and Heinegard, D. Cloning and sequence analysis of rat bone sialoprotein (osteopontin) cDNA reveals an Arg-Gly-Asp cell-binding sequence. Proc. Natl. Acad. Sci. U.S.A. 83 (23), 8819-8823 (1986)

NCBI and Uniprot Product Information

• NCBI GI #: 189405
• NCBI GeneID: 6696
• Molecular Weight: 33,843 Da
• NCBI Official Full Name: osteopontin
• NCBI Official Synonym Full Names: secreted phosphoprotein 1
• NCBI Official Symbol: SPP1
• NCBI Official Synonym Symbols: OPN; BNSP; BSPI; ETA-1
• NCBI Protein Information: osteopontin
• UniProt Protein Name: Osteopontin
• Protein Family: Osteopontin
• UniProt Gene Name: SPP1
• UniProt Synonym Gene Names: BNSP; OPN; SPP-1

NCBI Description

The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

osteopontin: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Belongs to the osteopontin family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q22.1

Cellular Component: endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi apparatus

Molecular Function: extracellular matrix binding; protein binding

Biological Process: androgen catabolic process; cell adhesion; cellular protein metabolic process; decidualization; embryo implantation; extracellular matrix disassembly; extracellular matrix organization and biogenesis; osteoblast differentiation; positive regulation of bone resorption; positive regulation of transcription, DNA-dependent; post-translational protein modification; response to vitamin D