Rabbit anti-Human Claudin 14 Polyclonal Antibody | anti-CLDN14 antibody

Rabbit anti Claudin 14 Polyclonal Antibody

Cat. Num. AAA460016

• Gene Names: CLDN14; DFNB29
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Epitope Affinity Purification
• Synonyms: Claudin 14; Polyclonal Antibody; Rabbit anti Claudin 14 Polyclonal Antibody; CLDN14; anti-CLDN14 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Epitope Affinity Purification
• Form/Format: Supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Sequence Length: 239

• Applicable Applications for anti-CLDN14 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: Immunohistochemistry: 2-10 ug/ml; Western Blot: 0.1-1 ug/ml
• Preparation: A synthetic peptide derived from C-terminus of human Claudin 14 protein
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20 degree C. The antibodies can be stored at 2 degree C - 8 degree C for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

References

Wilcox, E.R., et al Mutations in the gene encoding tight junction claudin-14 cause autosomal recessive deafness DFNB29. Cell 104 (1), 165-172 (2001)

NCBI and Uniprot Product Information

• NCBI GI #: 225703142
• NCBI GeneID: 23562
• NCBI Accession #: NP_001139551.1
• NCBI GenBank Nucleotide #: NM_001146079.1
• UniProt Accession #: O95500
• Molecular Weight: 25,699 Da
• NCBI Official Full Name: claudin-14
• NCBI Official Synonym Full Names: claudin 14
• NCBI Official Symbol: CLDN14
• NCBI Official Synonym Symbols: DFNB29
• NCBI Protein Information: claudin-14
• UniProt Protein Name: Claudin-14
• Protein Family: Claudin
• UniProt Gene Name: CLDN14

NCBI Description

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. The encoded protein also binds specifically to the WW domain of Yes-associated protein. Defects in this gene are the cause of an autosomal recessive form of nonsyndromic sensorineural deafness. It is also reported that four synonymous variants in this gene are associated with kidney stones and reduced bone mineral density. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jun 2010]

Uniprot Description

Claudin-14: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. Defects in CLDN14 are the cause of deafness autosomal recessive type 29 (DFNB29). DFNB29 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Belongs to the claudin family.

Protein type: Cell adhesion; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 21q22.13

Cellular Component: endoplasmic reticulum; plasma membrane; tight junction

Molecular Function: identical protein binding

Biological Process: calcium-independent cell-cell adhesion; protein complex assembly

Disease: Deafness, Autosomal Recessive 29