Human Fibulin 5 (FBLN5) RTU ELISA Kit | FBLN5 rtu elisa kit

Human Fibulin 5 (FBLN5) ELISA Kit

Cat. Num. AAA459223

• Gene Names: FBLN5; EVEC; UP50; ADCL2; ARMD3; DANCE; ARCL1A; FIBL-5; HNARMD
• Reactivity: Human
• Synonyms: Fibulin 5 (FBLN5); Human Fibulin 5 (FBLN5) ELISA Kit; ARMD3; DANCE; EVEC; UP50; FIBL-5; Dance; Developmental arteries and neural crest EGF-like protein; FBLN5 rtu elisa kit

• Reactivity: Human
• Sequence Length: 448

• Assay Type: Sandwich
• Samples: Serum, Plasma, Urine or Other Biological Fluids.
• Detection Range: 3.12-200ng/mL
• Sensitivity: 1.24ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FBLN5 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FBLN5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to FBLN5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FBLN5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FBLN5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 19743803
• NCBI GeneID: 10516
• NCBI Accession #: NP_006320.2
• NCBI GenBank Nucleotide #: NM_006329.3
• UniProt Accession #: Q9UBX5; O75966; Q6IAL4; Q6UWA3
• Molecular Weight: 50,180 Da
• NCBI Official Full Name: fibulin-5
• NCBI Official Synonym Full Names: fibulin 5
• NCBI Official Symbol: FBLN5
• NCBI Official Synonym Symbols: EVEC; UP50; ADCL2; ARMD3; DANCE; ARCL1A; FIBL-5; HNARMD
• NCBI Protein Information: fibulin-5
• UniProt Protein Name: Fibulin-5
• Protein Family: Fibulin
• UniProt Gene Name: FBLN5
• UniProt Synonym Gene Names: DANCE; FIBL-5; Dance; UP50

NCBI Description

The protein encoded by this gene is a secreted, extracellular matrix protein containing an Arg-Gly-Asp (RGD) motif and calcium-binding EGF-like domains. It promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. It is prominently expressed in developing arteries but less so in adult vessels. However, its expression is reinduced in balloon-injured vessels and atherosclerotic lesions, notably in intimal vascular smooth muscle cells and endothelial cells. Therefore, the protein encoded by this gene may play a role in vascular development and remodeling. Defects in this gene are a cause of autosomal dominant cutis laxa, autosomal recessive cutis laxa type I (CL type I), and age-related macular degeneration type 3 (ARMD3). [provided by RefSeq, Jul 2008]

Uniprot Description

Essential for elastic fiber formation, is involved in the assembly of continuous elastin (ELN) polymer and promotes the interaction of microfibrils and ELN (PubMed:18185537). Stabilizes and organizes elastic fibers in the skin, lung and vasculature (). Promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. Vascular ligand for integrin receptors which may play a role in vascular development and remodeling (PubMed:10428823). May act as an adapter that mediates the interaction between FBN1 and ELN (PubMed:17255108).