Dog Cholecystokinin A Receptor (CCKAR) RTU ELISA Kit | CCKAR rtu elisa kit

Canine Cholecystokinin A Receptor (CCKAR) ELISA Kit

Cat. Num. AAA458177

• Reactivity: Dog
• Synonyms: Cholecystokinin A Receptor (CCKAR); Canine Cholecystokinin A Receptor (CCKAR) ELISA Kit; CCK-A; CCK1; CCKRA; CCK1R; Cholecystokinin Type 1 Receptor; CCKAR rtu elisa kit

• Reactivity: Dog
• Sequence Length: 428

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.124ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCKAR rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CCKAR. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CCKAR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CCKAR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CCKAR in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 60460879
• NCBI GeneID: 488846
• NCBI Accession #: NP_001012664.1
• NCBI GenBank Nucleotide #: NM_001012646.2
• UniProt Accession #: Q5D0K2
• Molecular Weight: 47,836 Da
• NCBI Official Full Name: cholecystokinin receptor type A
• NCBI Official Synonym Full Names: cholecystokinin A receptor
• NCBI Official Symbol: CCKAR
• NCBI Protein Information: cholecystokinin receptor type A
• UniProt Protein Name: Cholecystokinin receptor type A
• Protein Family: Cholecystokinin receptor
• UniProt Gene Name: CCKAR
• UniProt Synonym Gene Names: CCK-A receptor; CCK-AR; CCK1-R

Uniprot Description

Receptor for cholecystokinin. Mediates pancreatic growth and enzyme secretion, smooth muscle contraction of the gall bladder and stomach. Has a 1000-fold higher affinity for CCK rather than for gastrin. It modulates feeding and dopamine-induced behavior in the central and peripheral nervous system. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system ().