Human Actinin Alpha 4 (ACTN4) RTU ELISA Kit | ACTN4 rtu elisa kit

Human Actinin Alpha 4 (ACTN4) ELISA Kit

Cat. Num. AAA457354

• Gene Names: ACTN4; FSGS; FSGS1; ACTININ-4
• Reactivity: Human
• Synonyms: Actinin Alpha 4 (ACTN4); Human Actinin Alpha 4 (ACTN4) ELISA Kit; FSGS; FSGS1; F-actin cross-linking protein; Non-muscle alpha-actinin 4; ACTN4 rtu elisa kit

• Reactivity: Human
• Sequence Length: 911

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.781-50ng/mL
• Sensitivity: 0.24ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ACTN4 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ACTN4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ACTN4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ACTN4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ACTN4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 12025678
• NCBI GeneID: 81
• NCBI Accession #: NP_004915.2
• NCBI GenBank Nucleotide #: NM_004924.5
• UniProt Accession #: O43707; O76048; A4K467; D6PXK4
• Molecular Weight: 59,579 Da
• NCBI Official Full Name: alpha-actinin-4 isoform 1
• NCBI Official Synonym Full Names: actinin alpha 4
• NCBI Official Symbol: ACTN4
• NCBI Official Synonym Symbols: FSGS; FSGS1; ACTININ-4
• NCBI Protein Information: alpha-actinin-4
• UniProt Protein Name: Alpha-actinin-4
• Protein Family: Alpha-actinin
• UniProt Gene Name: ACTN4

NCBI Description

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq, Jul 2008]

Uniprot Description

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (PubMed:15772161). Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions (). May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA (PubMed:22351778).