Human Phospholipase A2, Group IVD (PLA2G4D) ELISA Kit | PLA2G4D elisa kit

Human Phospholipase A2, Group IVD (PLA2G4D) ELISA Kit

Cat. Num. AAA455746

• Gene Names: PLA2G4D; cPLA2delta
• Reactivity: Human
• Synonyms: Phospholipase A2; Group IVD (PLA2G4D); Human Phospholipase A2; Group IVD (PLA2G4D) ELISA Kit; cPLA2delta; Cytosolic phospholipase A2 delta; PLA2G4D elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PLA2G4D. No significant cross-reactivity or interference between PLA2G4D and analogues was observed.
• Sequence Length: 818

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.064ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PLA2G4D were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PLA2G4D were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PLA2G4D elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PLA2G4D in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PLA2G4D. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PLA2G4D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PLA2G4D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PLA2G4D in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 116174754
• NCBI GeneID: 283748
• NCBI Accession #: NP_828848.3
• NCBI GenBank Nucleotide #: NM_178034.3
• UniProt Accession #: Q86XP0; Q8N176
• Molecular Weight: 80,930 Da
• NCBI Official Full Name: cytosolic phospholipase A2 delta
• NCBI Official Synonym Full Names: phospholipase A2 group IVD
• NCBI Official Symbol: PLA2G4D
• NCBI Official Synonym Symbols: cPLA2delta
• NCBI Protein Information: cytosolic phospholipase A2 delta
• UniProt Protein Name: Cytosolic phospholipase A2 delta
• Protein Family: Cytosolic phospholipase
• UniProt Gene Name: PLA2G4D
• UniProt Synonym Gene Names: cPLA2-delta

NCBI Description

The phospholipase A2 enzyme family, including PLA2G4D, catalyze the hydrolysis of glycerophospholipids at the sn-2 position and then liberate free fatty acids and lysophospholipids (Chiba et al., 2004 [PubMed 14709560]).[supplied by OMIM, Jun 2009]

Uniprot Description

Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position. Not arachidonic acid-specific but has linoleic acid-specific activity. May play a role in inflammation in psoriatic lesions.