Mouse Matrix Metalloproteinase 2 (MMP2) ELISA Kit | MMP2 elisa kit

Mouse Matrix Metalloproteinase 2 (MMP2) ELISA Kit

Cat. Num. AAA454416

• Gene Names: Mmp2; GelA; Clg4a; MMP-2
• Reactivity: Mouse
• Synonyms: Matrix Metalloproteinase 2 (MMP2); Mouse Matrix Metalloproteinase 2 (MMP2) ELISA Kit; CLG4; CLG4A; MMP-II; MONA; TBE-1; PEX; Gelatinase A; 72kDa Gelatinase; 72kDa Type IV Collagenase; MMP2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of MMP2. No significant cross-reactivity or interference between MMP2 and analogues was observed.
• Sequence Length: 662

• Samples: Serum, plasma, tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: <0.065ng/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MMP2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MMP2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MMP2 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of MMP2 in mouse serum, plasma, tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MMP2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MMP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MMP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MMP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6678902
• NCBI GeneID: 17390
• NCBI Accession #: NP_032636.1
• NCBI GenBank Nucleotide #: NM_008610.3
• UniProt Accession #: P33434
• Molecular Weight: 65,883 Da
• NCBI Official Full Name: 72 kDa type IV collagenase preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 2
• NCBI Official Symbol: Mmp2
• NCBI Official Synonym Symbols: GelA; Clg4a; MMP-2
• NCBI Protein Information: 72 kDa type IV collagenase
• UniProt Protein Name: 72 kDa type IV collagenase
• Protein Family: Matrix metalloproteinase
• UniProt Gene Name: Mmp2
• UniProt Synonym Gene Names: MMP-2

NCBI Description

This gene encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that are involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded preproprotein undergoes proteolytic processing to generate a mature, zinc-dependent endopeptidase enzyme that hydrolyzes collagens, gelatins, laminin, fibronectin and elastin. Mice lacking the encoded protein exhibit suppressed angiogenesis and attenuated features of human multicentric osteolysis with arthritis including abnormal skeletal and craniofacial development. [provided by RefSeq, Feb 2016]

Uniprot Description

Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues ().