Human Enolase, Neuron Specific (NSE) ELISA Kit | NSE elisa kit

Human Enolase, Neuron Specific (NSE) ELISA Kit

Cat. Num. AAA454176

• Gene Names: ENO2; NSE; HEL-S-279
• Reactivity: Human
• Synonyms: Enolase; Neuron Specific (NSE); Human Enolase; Neuron Specific (NSE) ELISA Kit; ENO2; Enolase 2; Gamma Enolase; 2-phospho-D-glycerate hydro-lyase; Neural enolase; NSE elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of NSE. No significant cross-reactivity or interference between NSE and analogues was observed.
• Sequence Length: 434

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.625-40ng/mL
• Sensitivity: 0.229ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NSE were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NSE were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Standard Curve/Testing Data

Related Product Information for NSE elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NSE in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NSE. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NSE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NSE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NSE in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 5803011
• NCBI GeneID: 2026
• NCBI Accession #: NP_001966.1
• NCBI GenBank Nucleotide #: NM_001975.2
• UniProt Accession #: P09104; Q96J33; B7Z2X9
• Molecular Weight: 42,707 Da
• NCBI Official Full Name: gamma-enolase
• NCBI Official Synonym Full Names: enolase 2
• NCBI Official Symbol: ENO2
• NCBI Official Synonym Symbols: NSE; HEL-S-279
• NCBI Protein Information: gamma-enolase
• UniProt Protein Name: Gamma-enolase
• UniProt Gene Name: ENO2
• UniProt Synonym Gene Names: NSE

NCBI Description

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]

Uniprot Description

Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival ().