Canine Brain Derived Neurotrophic Factor (BDNF) ELISA Kit | BDNF elisa kit

Canine Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

Cat. Num. AAA453689

• Reactivity: Canine
• Synonyms: Brain Derived Neurotrophic Factor (BDNF); Canine Brain Derived Neurotrophic Factor (BDNF) ELISA Kit; Neurotrophin; Abrineurin; BDNF elisa kit

• Reactivity: Canine
• Specificity: This assay has high sensitivity and excellent specificity for detection of BDNF. No significant cross-reactivity or interference between BDNF and analogues was observed.
• Sequence Length: 247

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.055ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level BDNF were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level BDNF were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BDNF elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of BDNF in canine serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to BDNF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to BDNF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BDNF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of BDNF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 55742819
• NCBI GeneID: 403461
• NCBI Accession #: NP_001002975.1
• NCBI GenBank Nucleotide #: NM_001002975.1
• UniProt Accession #: Q7YRB4; Q9BFH2
• Molecular Weight: 27,780 Da
• NCBI Official Full Name: brain-derived neurotrophic factor
• NCBI Official Symbol: BDNF
• NCBI Protein Information: brain-derived neurotrophic factor
• UniProt Protein Name: Brain-derived neurotrophic factor
• Protein Family: Brain-derived neurotrophic factor
• UniProt Gene Name: BDNF
• UniProt Synonym Gene Names: BDNF

Uniprot Description

During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS ().