Human TNF Receptor Associated Factor 3 (TRAF3) ELISA Kit | TRAF3 elisa kit

Human TNF Receptor Associated Factor 3 (TRAF3) ELISA Kit

Cat. Num. AAA451713

• Gene Names: TRAF3; CAP1; LAP1; CAP-1; CRAF1; IIAE5; CD40bp
• Reactivity: Human
• Synonyms: TNF Receptor Associated Factor 3 (TRAF3); Human TNF Receptor Associated Factor 3 (TRAF3) ELISA Kit; LAP1; CAP-1; CD40bp; CRAF1; CD40 receptor-associated factor 1; CD40-binding protein; LMP1-associated protein 1; TRAF3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of TRAF3. No significant cross-reactivity or interference between TRAF3 and analogues was observed.
• Sequence Length: 485

• Samples: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 78.125-5000pg/mL
• Sensitivity: 29pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TRAF3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TRAF3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TRAF3 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TRAF3 in human serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TRAF3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TRAF3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TRAF3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TRAF3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 313661483
• NCBI GeneID: 7187
• NCBI Accession #: NP_001186356.1
• NCBI GenBank Nucleotide #: NM_001199427.1
• UniProt Accession #: Q13114; Q12990; Q13076; Q13947; Q6AZX1; Q9UNL1; B7Z8C4
• Molecular Weight: 55,306 Da
• NCBI Official Full Name: TNF receptor-associated factor 3 isoform 3
• NCBI Official Synonym Full Names: TNF receptor associated factor 3
• NCBI Official Symbol: TRAF3
• NCBI Official Synonym Symbols: CAP1; LAP1; CAP-1; CRAF1; IIAE5; CD40bp
• NCBI Protein Information: TNF receptor-associated factor 3
• UniProt Protein Name: TNF receptor-associated factor 3
• Protein Family: TNF receptor-associated factor
• UniProt Gene Name: TRAF3
• UniProt Synonym Gene Names: CAP1; CRAF1; CRAF1; CD40BP; LAP1

NCBI Description

The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from, members of the TNF receptor (TNFR) superfamily. This protein participates in the signal transduction of CD40, a TNFR family member important for the activation of the immune response. This protein is found to be a critical component of the lymphotoxin-beta receptor (LTbetaR) signaling complex, which induces NF-kappaB activation and cell death initiated by LTbeta ligation. Epstein-Barr virus encoded latent infection membrane protein-1 (LMP1) can interact with this and several other members of the TRAF family, which may be essential for the oncogenic effects of LMP1. Several alternatively spliced transcript variants encoding three distinct isoforms have been reported. [provided by RefSeq, Dec 2010]

Uniprot Description

Regulates pathways leading to the activation of NF-kappa-B and MAP kinases, and plays a central role in the regulation of B-cell survival. Part of signaling pathways leading to the production of cytokines and interferon. Required for normal antibody isotype switching from IgM to IgG. Plays a role T-cell dependent immune responses. Plays a role in the regulation of antiviral responses. Is an essential constituent of several E3 ubiquitin-protein ligase complexes. May have E3 ubiquitin-protein ligase activity and promote 'Lys-63'-linked ubiquitination of target proteins. Inhibits activation of NF-kappa-B in response to LTBR stimulation. Inhibits TRAF2-mediated activation of NF-kappa-B. Down-regulates proteolytic processing of NFKB2, and thereby inhibits non-canonical activation of NF-kappa-B. Promotes ubiquitination and proteasomal degradation of MAP3K14.