Human Junctional Adhesion Molecule 3 (JAM3) ELISA Kit | JAM3 elisa kit

Human Junctional Adhesion Molecule 3 (JAM3) ELISA Kit

Cat. Num. AAA450721

• Gene Names: JAM3; JAMC; JAM-2; JAM-3; JAM-C
• Reactivity: Human
• Synonyms: Junctional Adhesion Molecule 3 (JAM3); Human Junctional Adhesion Molecule 3 (JAM3) ELISA Kit; JAM-C; JAMC; JAM3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of JAM3. No significant cross-reactivity or interference between JAM3 and analogues was observed.
• Sequence Length: 259

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 15.625-1000pg/mL
• Sensitivity: 6.2pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level JAM3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level JAM3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for JAM3 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of JAM3 in human serum, plasma and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to JAM3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to JAM3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain JAM3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of JAM3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 328927057
• NCBI GeneID: 83700
• NCBI Accession #: NP_001192258.1
• NCBI GenBank Nucleotide #: NM_001205329.1
• UniProt Accession #: Q9BX67; Q8WWL8; Q96FL1; B3KWG9
• Molecular Weight: 29,223 Da
• NCBI Official Full Name: junctional adhesion molecule C isoform 2
• NCBI Official Synonym Full Names: junctional adhesion molecule 3
• NCBI Official Symbol: JAM3
• NCBI Official Synonym Symbols: JAMC; JAM-2; JAM-3; JAM-C
• NCBI Protein Information: junctional adhesion molecule C
• UniProt Protein Name: Junctional adhesion molecule C
• Protein Family: Junctional adhesion molecule
• UniProt Gene Name: JAM3
• UniProt Synonym Gene Names: JAM-C; JAM-3

NCBI Description

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. Unlike other proteins in this family, the this protein is unable to adhere to leukocyte cell lines and only forms weak homotypic interactions. The encoded protein is a member of the junctional adhesion molecule protein family and acts as a receptor for another member of this family. A mutation in an intron of this gene is associated with hemorrhagic destruction of the brain, subependymal calcification, and congenital cataracts. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Apr 2011]

Uniprot Description

Participates in cell-cell adhesion. It is a counter-receptor for ITGAM, mediating leukocyte-platelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN). The soluble form is a mediator of angiogenesis.