Human Butyrophilin Subfamily 1, Member A1 (BTN1A1) ELISA Kit | BTN1A1 elisa kit

Human Butyrophilin Subfamily 1, Member A1 (BTN1A1) ELISA Kit

Cat. Num. AAA450421

• Gene Names: BTN1A1; BT; BTN; BTN1
• Reactivity: Human
• Synonyms: Butyrophilin Subfamily 1; Member A1 (BTN1A1); Human Butyrophilin Subfamily 1; Member A1 (BTN1A1) ELISA Kit; BTN1-A1; BT; BTN; BTN1A1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of BTN1A1. No significant cross-reactivity or interference between BTN1A1 and analogues was observed.
• Sequence Length: 526

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.057ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level BTN1A1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level BTN1A1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BTN1A1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of BTN1A1 in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to BTN1A1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to BTN1A1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BTN1A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of BTN1A1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 166197658
• NCBI GeneID: 696
• NCBI Accession #: NP_001723.2
• NCBI GenBank Nucleotide #: NM_001732.2
• UniProt Accession #: Q13410; Q4VAN3; Q4VAN4; Q9H458
• Molecular Weight: 58,960 Da
• NCBI Official Full Name: butyrophilin subfamily 1 member A1
• NCBI Official Synonym Full Names: butyrophilin subfamily 1 member A1
• NCBI Official Symbol: BTN1A1
• NCBI Official Synonym Symbols: BT; BTN; BTN1
• NCBI Protein Information: butyrophilin subfamily 1 member A1
• UniProt Protein Name: Butyrophilin subfamily 1 member A1
• Protein Family: Butyrophilin subfamily
• UniProt Gene Name: BTN1A1
• UniProt Synonym Gene Names: BTN; BT

NCBI Description

Butyrophilin is the major protein associated with fat droplets in the milk. It is a member of the immunoglobulin superfamily. It may have a cell surface receptor function. The human butyrophilin gene is localized in the major histocompatibility complex (MHC) class I region of 6p and may have arisen relatively recently in evolution by the shuffling of exons between 2 ancestral gene families [provided by RefSeq, Jul 2008]

Uniprot Description

May function in the secretion of milk-fat droplets. May act as a specific membrane-associated receptor for the association of cytoplasmic droplets with the apical plasma membrane (). Inhibits the proliferation of CD4 and CD8 T-cells activated by anti-CD3 antibodies, T-cell metabolism and IL2 and IFNG secretion ().