Mouse Surfactant Associated Protein B (SPB) RTU ELISA Kit | SPB rtu elisa kit

Mouse Surfactant Associated Protein B (SPB) RTU ELISA Kit

Cat. Num. AAA4503862

• Gene Names: ENTPD1; CD39; SPG64; ATPDase; NTPDase-1
• Reactivity: Mouse
• Purity: Affinity Purification
• Synonyms: Surfactant Associated Protein B (SPB); Mouse Surfactant Associated Protein B (SPB) RTU ELISA Kit; SFTPB; PSPB; SFTB3; SFTP3; SP-B; Pulmonary Surfactant Protein B; 18 kDa pulmonary-surfactant protein; 6 kDa protein; SPB rtu elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of SPB. No significant cross-reactivity or interference between SPB and analogues was observed.
• Purity/Purification: Affinity Purification
• Sequence Length: 517

• Samples: Mouse Serum, Plasma, Tissue Homogenates Or Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.781-50ng/mL
• Sensitivity: 0.33ng/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SPB were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SPB were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SPB rtu elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of SPB in mouse serum, plasma, tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SPB. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SPB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SPB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SPB in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 147905700
• NCBI GeneID: 953
• NCBI Accession #: NP_001091645.1
• NCBI GenBank Nucleotide #: NM_001098175.1
• UniProt Accession #: P49961; Q5T561; Q5T562; Q86VV3; Q9UQQ9; Q9Y3Q9; A9Z1X8; B4DWB9; B4E1X1; B7Z599; G3XAF6
• Molecular Weight: 58,706 Da
• NCBI Official Full Name: ectonucleoside triphosphate diphosphohydrolase 1 isoform 2
• NCBI Official Synonym Full Names: ectonucleoside triphosphate diphosphohydrolase 1
• NCBI Official Symbol: ENTPD1
• NCBI Official Synonym Symbols: CD39; SPG64; ATPDase; NTPDase-1
• NCBI Protein Information: ectonucleoside triphosphate diphosphohydrolase 1
• UniProt Protein Name: Ectonucleoside triphosphate diphosphohydrolase 1
• UniProt Gene Name: ENTPD1
• UniProt Synonym Gene Names: CD39; NTPDase 1; Ecto-ATPDase 1; Ecto-ATPase 1

NCBI Description

The protein encoded by this gene is a plasma membrane protein that hydrolyzes extracellular ATP and ADP to AMP. Inhibition of this protein's activity may confer anticancer benefits. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2015]

Uniprot Description

In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation by hydrolyzing platelet-activating ADP to AMP. Hydrolyzes ATP and ADP equally well.