Human 14-3-3 Protein Epsilon (YWHAE) ELISA Kit | YWHAE elisa kit

Human 14-3-3 Protein Epsilon (YWHAE) ELISA Kit

Cat. Num. AAA4503830

• Gene Names: YWHAE; MDS; HEL2; MDCR; KCIP-1; 14-3-3E
• Reactivity: Human
• Synonyms: 14-3-3 Protein Epsilon (YWHAE); Human 14-3-3 Protein Epsilon (YWHAE) ELISA Kit; YWHAE elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of YWHAE. No significant cross-reactivity or interference between YWHAE and analogues was observed.
• Sequence Length: 255

• Samples: Human Serum, Plasma, Tissue Homogenates Or Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.1-5000pg/mL
• Sensitivity: 32.9pg/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level YWHAE were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level YWHAE were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: Stable for 12 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for YWHAE elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of YWHAE in human serum, plasma, tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to YWHAE. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to YWHAE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain YWHAE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of YWHAE in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 5803225
• NCBI GeneID: 7531
• NCBI Accession #: NP_006752.1
• NCBI GenBank Nucleotide #: NM_006761.4
• UniProt Accession #: P62258; P29360; P42655; Q4VJB6; Q53XZ5; Q63631; Q7M4R4; B3KY71; D3DTH5
• Molecular Weight: 26,504 Da
• NCBI Official Full Name: 14-3-3 protein epsilon
• NCBI Official Synonym Full Names: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon
• NCBI Official Symbol: YWHAE
• NCBI Official Synonym Symbols: MDS; HEL2; MDCR; KCIP-1; 14-3-3E
• NCBI Protein Information: 14-3-3 protein epsilon
• UniProt Protein Name: 14-3-3 protein epsilon
• Protein Family: 14-3-3 protein
• UniProt Gene Name: YWHAE
• UniProt Synonym Gene Names: 14-3-3E

NCBI Description

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner (). Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm (PubMed:12917326).