Human Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) RTU ELISA Kit | SMUG1 rtu elisa kit

Human Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) ELISA Kit

Cat. Num. AAA4502563

• Gene Names: SMUG1; FDG; UNG3; HMUDG
• Reactivity: Human
• Synonyms: Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1); Human Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) ELISA Kit; FDG; HMUDG; UNG3; SMUG1 rtu elisa kit

• Reactivity: Human
• Sequence Length: 270

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.047ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SMUG1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SMUG1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SMUG1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SMUG1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SMUG1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 344925869
• NCBI GeneID: 23583
• NCBI Accession #: NP_001230716.1
• NCBI GenBank Nucleotide #: NM_001243787.1
• UniProt Accession #: Q53HV7; O95862; Q0D2M0; Q8NB71; Q9BWC8; A8K2K9
• Molecular Weight: 19,627 Da
• NCBI Official Full Name: single-strand selective monofunctional uracil DNA glycosylase isoform 1
• NCBI Official Synonym Full Names: single-strand-selective monofunctional uracil-DNA glycosylase 1
• NCBI Official Symbol: SMUG1
• NCBI Official Synonym Symbols: FDG; UNG3; HMUDG
• NCBI Protein Information: single-strand selective monofunctional uracil DNA glycosylase
• UniProt Protein Name: Single-strand selective monofunctional uracil DNA glycosylase
• Protein Family: Single-strand selective monofunctional uracil DNA glycosylase
• UniProt Gene Name: SMUG1

NCBI Description

This gene encodes a protein that participates in base excision repair by removing uracil from single- and double-stranded DNA. Many alternatively spliced transcript variants exist for this gene; the full-length nature is known for some but not all of the variants. [provided by RefSeq, Aug 2011]

Uniprot Description

Recognizes base lesions in the genome and initiates base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA with a preference for single-stranded DNA substrates. The activity is greater toward mismatches (U/G) compared to matches (U/A). Excises uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5-hydroxymethyluracil (hmU)] in ssDNA and dsDNA, but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine), nor other oxidized bases. The activity is damage-specific and salt-dependent. The substrate preference is the following: ssDNA > dsDNA (G pair) = dsDNA (A pair) at low salt concentration, and dsDNA (G pair) > dsDNA (A pair) > ssDNA at high salt concentration.