Regenerating Islet Derived Protein 3 Alpha (REG3a) RTU ELISA Kit | REG3a rtu elisa kit
Rat Regenerating Islet Derived Protein 3 Alpha (REG3a) ELISA Kit
Gene Names
Reg3a; Pap2; PapII
Reactivity
Rat
Synonyms
Regenerating Islet Derived Protein 3 Alpha (REG3a); Rat Regenerating Islet Derived Protein 3 Alpha (REG3a) ELISA Kit; HIP; INGAP; PAP-H; PAP1; PBCGF; REG-III; REG3; Pancreatitis-Associated Protein; Hepatointestinal pancreatic protein; Human proislet peptide; Pancreatitis-associated protein 1; REG3a rtu elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of REG3a. No significant cross-reactivity or interference between REG3a and analogues was observed.
Sequence Length
174
Samples
Serum, plasma or other biological fluids
Assay Type
Quantitative Competitive
Detection Range
6.25-400pg/mL
Sensitivity
< 2.34pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level REG3a were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level REG3a were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
Related Product Information for REG3a rtu elisa kit
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of REG3a in rat serum, plasma or other biological fluids.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to REG3a has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled REG3a and unlabeled REG3a (Standards or samples) with the pre-coated antibody specific to REG3a. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of REG3a in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of REG3a in the sample.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to REG3a has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled REG3a and unlabeled REG3a (Standards or samples) with the pre-coated antibody specific to REG3a. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of REG3a in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of REG3a in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
19,599 Da
NCBI Official Full Name
regenerating islet-derived protein 3-alpha
NCBI Official Synonym Full Names
regenerating islet-derived 3 alpha
NCBI Official Symbol
Reg3a
NCBI Official Synonym Symbols
Pap2; PapII
NCBI Protein Information
regenerating islet-derived protein 3-alpha
UniProt Protein Name
Regenerating islet-derived protein 3-alpha
Protein Family
UniProt Gene Name
Reg3a
UniProt Synonym Gene Names
Pap2; Reg3; REG-3-alpha; REG 3; Reg III-alpha
NCBI Description
lectin-related secretory protein; overexpressed during the acute phase of pancreatitis [RGD, Feb 2006]
Uniprot Description
Bactericidal C-type lectin. Regulates keratinocyte proliferation and differentiation after skin injury via activation of EXTL3-PI3K-AKT signaling pathway ().
Research Articles on REG3a
Similar Products
Product Notes
The Rat REG3a reg3a (Catalog #AAA4502205) is a RTU ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA4502205 RTU ELISA Kit recognizes Rat REG3a. It is sometimes possible for the material contained within the vial of "Regenerating Islet Derived Protein 3 Alpha (REG3a), RTU ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
