Rat Protein Kinase C Zeta (PKCz) RTU ELISA Kit | PKCz rtu elisa kit

Rat Protein Kinase C Zeta (PKCz) ELISA Kit

Cat. Num. AAA4501875

• Gene Names: Prkcz; Pkcz; r14-3-3; 14-3-3-zetaisoform
• Reactivity: Rat
• Synonyms: Protein Kinase C Zeta (PKCz); Rat Protein Kinase C Zeta (PKCz) ELISA Kit; PKC2; PKC-Z; PRKCZ; Protein Kinase M Zeta; PKCz rtu elisa kit

• Reactivity: Rat
• Sequence Length: 592

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.062ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PKCz rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PKCz. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PKCz. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PKCz, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PKCz in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 11968080
• NCBI GeneID: 25522
• NCBI Accession #: NP_071952.1
• NCBI GenBank Nucleotide #: NM_022507.1
• UniProt Accession #: P09217
• Molecular Weight: 67,733 Da
• NCBI Official Full Name: protein kinase C zeta type
• NCBI Official Synonym Full Names: protein kinase C, zeta
• NCBI Official Symbol: Prkcz
• NCBI Official Synonym Symbols: Pkcz; r14-3-3; 14-3-3-zetaisoform
• NCBI Protein Information: protein kinase C zeta type
• UniProt Protein Name: Protein kinase C zeta type
• UniProt Gene Name: Prkcz
• UniProt Synonym Gene Names: Pkcz

NCBI Description

may play an important role in insulin resistance in skeletal muscle and adipose tissue; mediates matrix metalloproteinase-9 (MMP-9) expression in glioma cells, key process in tumor invasion, through NF-kappaB [RGD, Feb 2006]

Uniprot Description

Calcium- and diacylglycerol-independent serine/threonine-protein kinase that functions in phosphatidylinositol 3-kinase (PI3K) pathway and mitogen-activated protein (MAP) kinase cascade, and is involved in NF-kappa-B activation, mitogenic signaling, cell proliferation, cell polarity, inflammatory response and maintenance of long-term potentiation (LTP). Upon lipopolysaccharide (LPS) treatment in macrophages, or following mitogenic stimuli, functions downstream of PI3K to activate MAP2K1/MEK1-MAPK1/ERK2 signaling cascade independently of RAF1 activation. Required for insulin-dependent activation of AKT3, but may function as an adapter rather than a direct activator. Upon insulin treatment may act as a downstream effector of PI3K and contribute to the activation of translocation of the glucose transporter SLC2A4/GLUT4 and subsequent glucose transport in adipocytes. In EGF-induced cells, binds and activates MAP2K5/MEK5-MAPK7/ERK5 independently of its kinase activity and can activate JUN promoter through MEF2C. Through binding with SQSTM1/p62, functions in interleukin-1 signaling and activation of NF-kappa-B with the specific adapters RIPK1 and TRAF6. Participates in TNF-dependent transactivation of NF-kappa-B by phosphorylating and activating IKBKB kinase, which in turn leads to the degradation of NF-kappa-B inhibitors. In migrating astrocytes, forms a cytoplasmic complex with PARD6A and is recruited by CDC42 to function in the establishment of cell polarity along with the microtubule motor and dynein. In association with FEZ1, stimulates neuronal differentiation in PC12 cells. In the inflammatory response, is required for the T-helper 2 (Th2) differentiation process, including interleukin production, efficient activation of JAK1 and the subsequent phosphorylation and nuclear translocation of STAT6. May be involved in development of allergic airway inflammation (asthma), a process dependent on Th2 immune response. In the NF-kappa-B-mediated inflammatory response, can relieve SETD6-dependent repression of NF-kappa-B target genes by phosphorylating the RELA subunit at 'Ser-311'. Necessary and sufficient for LTP maintenance in hippocampal CA1 pyramidal cells. In vein endothelial cells treated with the oxidant peroxynitrite, phosphorylates STK11 leading to nuclear export of STK11, subsequent inhibition of PI3K/Akt signaling, and increased apoptosis. Phosphorylates VAMP2 in vitro ().