Mouse Leukocyte Cell Derived Chemotaxin 2 (LECT2) RTU ELISA Kit | LECT2 rtu elisa kit
Mouse Leukocyte Cell Derived Chemotaxin 2 (LECT2) ELISA Kit
Reactivity
Mouse
Synonyms
Leukocyte Cell Derived Chemotaxin 2 (LECT2); Mouse Leukocyte Cell Derived Chemotaxin 2 (LECT2) ELISA Kit; Chm-II; Chm2; LECT2 rtu elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of LECT2. No significant cross-reactivity or interference between LECT2 and analogues was observed.
Sequence Length
151
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
31.2-2000pg/mL
Sensitivity
< 13.6pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LECT2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LECT2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for LECT2 rtu elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of LECT2 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LECT2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to LECT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LECT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of LECT2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LECT2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to LECT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LECT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of LECT2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
14,586 Da
NCBI Official Full Name
leukocyte cell-derived chemotaxin-2
NCBI Official Synonym Full Names
leukocyte cell-derived chemotaxin 2
NCBI Official Symbol
Lect2
NCBI Protein Information
leukocyte cell-derived chemotaxin-2
UniProt Protein Name
Leukocyte cell-derived chemotaxin-2
Protein Family
UniProt Gene Name
Lect2
UniProt Synonym Gene Names
LECT-2; ChM-II
Uniprot Description
Has a neutrophil chemotactic activity. Also a positive regulator of chondrocyte proliferation.
